ThiM

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  • Description: hydroxyethylthiazole kinase

Gene name thiM
Synonyms thiK, ipa-25d, ywbJ
Essential no
Product hydroxyethylthiazole kinase
Function biosynthesis of thiamine
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 28 kDa, 5.393
Gene length, protein length 816 bp, 272 aa
Immediate neighbours thiE, ywbI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThiM context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU38300

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole (according to Swiss-Prot)
  • Protein family: Thz kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

N Campobasso, I I Mathews, T P Begley, S E Ealick
Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution.
Biochemistry: 2000, 39(27);7868-77
[PubMed:10891066] [WorldCat.org] [DOI] (P p)

Y Zhang, S V Taylor, H J Chiu, T P Begley
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.
J Bacteriol: 1997, 179(9);3030-5
[PubMed:9139923] [WorldCat.org] [DOI] (P p)