thiG

168

hydroxyethylthiazole phosphate biosynthesis

locus: BSU_11690

Molecular weight: 26.87 kDa

pI: 4.72

Protein length: 256 aa

Gene length: 771 bp

function: biosynthesis of thiamine

product: thiamin thiazole synthase

essential: no

ec: null

synonyms: thiG, yjbT

List of homologs in different organisms, belongs to COG2022 (Galperin et al., 2021)

Gene

Coordinates: 1,245,041 → 1,245,811

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The protein

Catalyzed reaction/ biological activity

• 1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH₂-C(O)SH --> 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS]-C-terminal Gly-Gly + 2 H⁺ + 2 H₂O (according to UniProt)

Protein family

• ThiG family (single member, according to UniProt)

Structure

• [PDB|1XM3], [PDB|1TYG] (complex with [protein|95DA3F899037140F48B99E065C768356CE08FA94|thiS]) [Pubmed|15362849]
• [AF|O31618]

Modification

• phosphorylated on ser/ thr/ tyr [Pubmed|16493705], [Pubmed|17726680]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|47E47FB7D9C079DF603906694C4649A049814346|tenA]-[gene|CA6AAE8AC5B5EF01A911B035F8782579CD876C08|tenI]-[gene|87B5C84B7EE10F60B545CCFEF6A527A546B80432|thiO]-[gene|95DA3F899037140F48B99E065C768356CE08FA94|thiS]-[gene|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]-[gene|CCD3DDE9E52FCD4906735059CF30593BEB56FD1B|thiF]-[gene|97EDB4ACD64C5FA2E6014ED133DF18F602FF58BB|thiD]

  description: [Pubmed|1898926]

  regulation

  • repressed by thiamine ([wiki|Thi-box]) [Pubmed|16356850]
  • the [wiki|Thi-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, via [wiki|RNA switch], in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

Biological materials

Mutant

• MGNA-B165 (yjbT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1164 NBRP B. subtilis, Japan]
• BKE11690 (Δ[gene|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCTCATCCGCCTCCTACAAA,  downstream forward: _UP4_GTATGACAGGCAGGTATTCA
• BKK11690 (Δ[gene|B58B8F260E446E28BC2E3A9AA84EA771EC0A8634|thiG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11690 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCTCATCCGCCTCCTACAAA,  downstream forward: _UP4_GTATGACAGGCAGGTATTCA

References

Reviews

• 19348578,10382260

Original Publications

• 15362849,17726680,16493705,19216519,14567704