ThiF

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  • Description: hydroxyethylthiazole phosphate biosynthesis

Gene name thiF
Synonyms yjbU
Essential no
Product unknown
Function biosynthesis of thiamine
Gene expression levels in SubtiExpress: thiF
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 36 kDa, 5.619
Gene length, protein length 1008 bp, 336 aa
Immediate neighbours thiG, thiD
Sequences Protein DNA DNA_with_flanks
Genetic context
ThiF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThiF expression.png




























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Thi-box

The gene

Basic information

  • Locus tag: BSU11700

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): MoeB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1ZFN (from E. coli, 35% identity, 51% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)

Original publications

Narasimhan Sudarsan, Smadar Cohen-Chalamish, Shingo Nakamura, Gail Mitchell Emilsson, Ronald R Breaker
Thiamine pyrophosphate riboswitches are targets for the antimicrobial compound pyrithiamine.
Chem Biol: 2005, 12(12);1325-35
[PubMed:16356850] [WorldCat.org] [DOI] (P p)

David M Duda, Helen Walden, John Sfondouris, Brenda A Schulman
Structural analysis of Escherichia coli ThiF.
J Mol Biol: 2005, 349(4);774-86
[PubMed:15896804] [WorldCat.org] [DOI] (P p)

Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704] [WorldCat.org] [DOI] (P p)

A S Pang, S Nathoo, S L Wong
Cloning and characterization of a pair of novel genes that regulate production of extracellular enzymes in Bacillus subtilis.
J Bacteriol: 1991, 173(1);46-54
[PubMed:1898926] [WorldCat.org] [DOI] (P p)