Difference between revisions of "ThiC"

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(Database entries)
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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin synthesis]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 65 kDa, 5.262   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 65 kDa, 5.262   

Revision as of 09:53, 16 February 2010

  • Description: biosynthesis of the pyrimidine moiety of thiamine

Gene name thiC
Synonyms thiA
Essential no
Product unknown
Function biosynthesis of thiamine
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin synthesis
MW, pI 65 kDa, 5.262
Gene length, protein length 1770 bp, 590 aa
Immediate neighbours ygaJ, ygaK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThiC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU08790

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: thiC family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Operon:
  • Regulation:
    • repressed by thiamine and 2-methyl-4-amino-5-hydroxymethylpyrimidine PubMed
  • Regulatory mechanism:
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685] [WorldCat.org] [DOI] (P p)

Y Zhang, T P Begley
Cloning, sequencing and regulation of thiA, a thiamin biosynthesis gene from Bacillus subtilis.
Gene: 1997, 198(1-2);73-82
[PubMed:9370266] [WorldCat.org] [DOI] (P p)