Difference between revisions of "ThdF"

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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
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* '''Operon:''' ''[[thdF]]-[[gidA]]-[[rsmG]]-[[noc]]''
  
 
* '''Sigma factor:'''  
 
* '''Sigma factor:'''  

Revision as of 10:43, 13 July 2009

  • Description: GTP-binding protein, putative tRNA modification GTPase

Gene name thdF
Synonyms
Essential no
Product putative tRNA modification GTPase
Function tRNA modification
MW, pI 50 kDa, 4.643
Gene length, protein length 1377 bp, 459 aa
Immediate neighbours gidA, jag
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThdF jag spoIIIJ rnpA rpmH context.png
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU41020

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: MnmE subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)