TcyA

• Description: cystine ABC transporter (binding protein)
  
  

Gene name	tcyA
Synonyms	yckK
Essential	no
Product	cystine ABC transporter (binding protein)
Function	cystine uptake
Gene expression levels in SubtiExpress: tcyA
Interactions involving this protein in SubtInteract: TcyA
Metabolic function and regulation of this protein in SubtiPathways: TcyA
MW, pI	29 kDa, 9.118
Gene length, protein length	804 bp, 268 aa
Immediate neighbours	tcyB, bsdA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, biosynthesis/ acquisition of amino acids, membrane proteins

The gene

Basic information

• Locus tag: BSU03610

Database entries

• BsubCyc: BSU03610

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TcyC-TcyB-TcyA PubMed

• Localization:
  • attached to the membrane via TcyB PubMed

Database entries

• BsubCyc: BSU03610

• Structure:

• UniProt: P42199

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: tcyA-tcyB-tcyC (putative) PubMed

• Expression browser: tcyA PubMed

• Sigma factor:

• Regulation:
  • very weakly expressed under all conditions PubMed
  • strongly repressed in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Imke G de Jong, Jan-Willem Veening, Oscar P Kuipers
Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation.
Environ Microbiol: 2012, 14(12);3110-21
[PubMed:23033921] [WorldCat.org] [DOI] (I p)

Pierre Burguière, Sandrine Auger, Marie-Françoise Hullo, Antoine Danchin, Isabelle Martin-Verstraete
Three different systems participate in L-cystine uptake in Bacillus subtilis.
J Bacteriol: 2004, 186(15);4875-84
[PubMed:15262924] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

F Rodriguez, G Grandi
An operon encoding a novel ABC-type transport system in Bacillus subtilis.
Microbiology (Reading): 1995, 141 ( Pt 7);1781-4
[PubMed:7551042] [WorldCat.org] [DOI] (P p)