tasA

168

major component of biofilm matrix, forms bundles of fibres

locus: BSU_24620

Molecular weight: 28.15 kDa

pI: 5.44

Protein length: 261 aa

Gene length: 786 bp

function: [wiki|biofilm formation]

product: major component of biofilm matrix

essential: no

synonyms: tasA, cotN, yqhF

List of homologs in different organisms, belongs to COG5884 (Galperin et al., 2021)

Gene

Coordinates: 2,553,081 → 2,553,866

Phenotypes of a mutant

• altered cell death pattern in colonies [Pubmed|23012477]
• increases membrane fluidity and cell death [pubmed|32313019]
• failure to produce structured and wrinkled colonies [pubmed|33803642]

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The protein

Catalyzed reaction/ biological activity

• forms amyloid fibers that bind cells together in the biofilm [Pubmed|20080671]

Protein family

• peptidase M73 family (single member, according to UniProt)

Structure

• [PDB|5OF1] [pubmed|29531041]
• [PDB|5OF2] [pubmed|29531041]
• [AF|P54507]

[wiki|Localization]

• extracellular (signal peptide) [Pubmed|18957862], secretion requires [protein|09B082BF39703F0D9A5980E643175DBD59F5B228|sipW] [Pubmed|10049401]

Expression and Regulation

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Mutant

• MGNA-C449 (yqhF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2447 NBRP B. subtilis, Japan]
• BKE24620 (Δ[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGCTCCCCTTTTA,  downstream forward: _UP4_TAATAACAGCAAAAAAAAGA
• BKK24620 (Δ[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGCTCCCCTTTTA,  downstream forward: _UP4_TAATAACAGCAAAAAAAAGA

References

Reviews

• 20735481,20351052,21488983,24988880,25907113,26418850,28783117,30098341,33239434,33824496,37108873

Original Publications

• 23352144,23352134,23632024,23012477,21815947,21856853,18047568,16430695,16430696,10368135,18430133,18647168,10464223,17720793,18957862,12107147,20080671,10049401,23646920,24488317,25894589,26883633,29531041,29565118,29590605,29802781,29887307,29921978,30297741,30833350,31370706,32313019,32430292,32491277,32753507,32779018,33033811,33803642,33806534,33803642,34492253,35098894,36330326,36400765,37068239,37739955,38351711