tasA
168
major component of biofilm matrix, forms bundles of fibres
locus
BSU_24620
Molecular weight
28.15 kDa
pI
5.44
function
[wiki|biofilm formation]
product
major component of biofilm matrix
essential
no
synonyms
tasA, cotN, yqhF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5884 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,553,081 → 2,553,866
Phenotypes of a mutant
altered cell death pattern in colonies [Pubmed|23012477]
increases membrane fluidity and cell death [pubmed|32313019]
failure to produce structured and wrinkled colonies [pubmed|33803642]
The protein
Catalyzed reaction/ biological activity
forms amyloid fibers that bind cells together in the biofilm [Pubmed|20080671]
Protein family
peptidase M73 family (single member, according to UniProt)
Structure
[PDB|5OF1] [pubmed|29531041]
[PDB|5OF2] [pubmed|29531041]
[AF|P54507]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862], secretion requires [protein|09B082BF39703F0D9A5980E643175DBD59F5B228|sipW] [Pubmed|10049401]
Expression and Regulation
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-C449 (yqhF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2447 NBRP B. subtilis, Japan]
BKE24620 (Δ[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGCTCCCCTTTTA, downstream forward: _UP4_TAATAACAGCAAAAAAAAGA
BKK24620 (Δ[gene|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTAAGCTCCCCTTTTA, downstream forward: _UP4_TAATAACAGCAAAAAAAAGA
References
Reviews
Original Publications
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Time of last update: 2024-04-24 10:32:20
Author of last update: Jstuelk