Difference between revisions of "TasA"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
(Biological materials)
Line 137: Line 137:
 
=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutants:'''
 
** 1S121 (''tasA''::''spec''), {{PubMed|10049401}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S121&Search=1S121 BGSC]
 
** 1S121 (''tasA''::''spec''), {{PubMed|10049401}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S121&Search=1S121 BGSC]
 
** GP1571 (cat), available in [[ Jörg Stülke]]'s lab
 
** GP1571 (cat), available in [[ Jörg Stülke]]'s lab
 +
** GP1663 (deletion of ''[[yqhG]]-[[sinI]]-[[sinR]]-[[tasA]]''), available in [[ Jörg Stülke]]'s lab
 +
** GP1672 (deletion of ''[[sinR]]-[[tasA]]''::''cat'') {{PubMed|24493247}}, available in [[ Jörg Stülke]]'s lab
 
** GP1819 (aphA3), available in [[ Jörg Stülke]]'s lab
 
** GP1819 (aphA3), available in [[ Jörg Stülke]]'s lab
** GP1672 (deletion of ''[[sinR]]-[[tasA]]''::''cat'') {{PubMed|24493247}}, available in [[ Jörg Stülke]]'s lab
 
** GP1663 (deletion of ''[[yqhG]]-[[sinI]]-[[sinR]]-[[tasA]]''), available in [[ Jörg Stülke]]'s lab
 
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 17:19, 30 September 2014

  • Description: major component of biofilm matrix, forms amyloid fibers

Gene name tasA
Synonyms cotN, yqhF
Essential no
Product major component of biofilm matrix
Function biofilm formation
Gene expression levels in SubtiExpress: tasA
Interactions involving this protein in SubtInteract: TasA
Regulation of this protein in SubtiPathways:
tasA
MW, pI 28 kDa, 5.442
Gene length, protein length 783 bp, 261 aa
Immediate neighbours sinR, sipW
Sequences Protein DNA DNA_with_flanks
Genetic context
TasA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TasA expression.png















Categories containing this gene/protein

biofilm formation

This gene is a member of the following regulons

AbrB regulon, RemA regulon, SinR regulon

The gene

Basic information

  • Locus tag: BSU24620

Phenotypes of a mutant

  • altered cell death pattern in colonies PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: forms amyloid fibers that bind cells together in the biofilm PubMed
  • Protein family: peptidase M73 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • induction by sequestration of SinR by SinI or SlrA PubMed or by SlrR PubMed
    • the tapA-sipW-tasA operon is not expressed in a ymdB mutant PubMed
    • the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 199 PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original publications