Difference between revisions of "TapA"

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(Biological materials)
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
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* '''YFP fusion:''' GP1537 lacA::p(tapA-yfp ermC)
  
 
* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  

Revision as of 16:56, 27 January 2014

  • Description: required for the anchoring of the TasA amyloid fibers to the cell and for the initiation of fiber polymerization, minor fiber component



Gene name tapA
Synonyms yqhD, yqxM
Essential no
Product TasA anchoring/assembly protein
Function biofilm formation
Gene expression levels in SubtiExpress: tapA
Interactions involving this protein in SubtInteract: TapA
Regulation of this protein in SubtiPathways:
tapA
MW, pI 28 kDa, 6.677
Gene length, protein length 759 bp, 253 aa
Immediate neighbours sipW, yqzG
Sequences Protein DNA DNA_with_flanks
Genetic context
YqxM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TapA expression.png















Categories containing this gene/protein

biofilm formation, membrane proteins

This gene is a member of the following regulons

AbrB regulon, RemA regulon, SinR regulon

The gene

Basic information

  • Locus tag: BSU24640

Phenotypes of a mutant

The mutants are able to form a biofilm in the presence of D-amino acids PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: D-amino acids lead to disappearance of TapA from the cell wall PubMed
  • Localization:
    • attached to the cell surface (on the outside of the cell), associated with peptidoglycan PubMed
    • secretion requires SipW PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP1926 (in pAC6), available in Stülke lab
  • GFP fusion:
  • YFP fusion: GP1537 lacA::p(tapA-yfp ermC)
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Adam Driks
Tapping into the biofilm: insights into assembly and disassembly of a novel amyloid fibre in Bacillus subtilis.
Mol Microbiol: 2011, 80(5);1133-6
[PubMed:21488983] [WorldCat.org] [DOI] (I p)

Original publications