TagE

• Description: UDP-glucose:polyglycerol phosphate glucosyltransferase
  
  

Gene name	tagE
Synonyms	rodD, gtaA, gtaD
Essential	no
Product	UDP-glucose:polyglycerol phosphate glucosyltransferase
Function	biosynthesis of teichoic acid
MW, pI	78 kDa, 6.026
Gene length, protein length	2019 bp, 673 aa
Immediate neighbours	tagF, tagD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35730

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-glucose + poly(glycerol phosphate) = UDP + O-(alpha-D-glucosyl)poly(glycerol phosphate) (according to Swiss-Prot)

• Protein family: glycosyltransferase 1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-2 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P13484

• KEGG entry: [3]

• E.C. number: 2.4.1.52

Expression and regulation

• Operon: tagD-tagE-tagF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by phosphate (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Alistair Howell, Sarah Dubrac, Kasper Krogh Andersen, David Noone, Juliette Fert, Tarek Msadek, Kevin Devine
Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach.
Mol Microbiol: 2003, 49(6);1639-55
[PubMed:12950927] [WorldCat.org] [DOI] (P p)

W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886] [WorldCat.org] [DOI] (P p)

C Mauël, A Bauduret, C Chervet, S Beggah, D Karamata
In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes.
Microbiology (Reading): 1995, 141 ( Pt 10);2379-89
[PubMed:7581998] [WorldCat.org] [DOI] (P p)

A L Honeyman, G C Stewart
The nucleotide sequence of the rodC operon of Bacillus subtilis.
Mol Microbiol: 1989, 3(9);1257-68
[PubMed:2507871] [WorldCat.org] [DOI] (P p)