Difference between revisions of "SucD"

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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P80865 P80865]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P80865 P80865]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU16100]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16100]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.2.1.5 6.2.1.5]  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/6.2.1.5 6.2.1.5]  

Revision as of 04:55, 25 June 2009

  • Description: succinyl-CoA synthetase (alpha subunit)

Gene name sucD
Synonyms
Essential no
Product succinyl-CoA synthetase (alpha subunit)
Function TCA cycle
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 31 kDa, 5.587
Gene length, protein length 900 bp, 300 aa
Immediate neighbours sucC, smf
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SucD context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU16100

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA (according to Swiss-Prot)
  • Protein family: succinate/malate CoA ligase alpha subunit family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: Reversible Michaelis-Menten FEBS Letters
  • Domains:
  • Modification: phosphorylation on (Ser-19 OR Thr-20) PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1JKJ (E. coli)
  • KEGG entry: [3]

Additional information

The enzyme is a dimer FEBS Letters

Expression and regulation

  • Sigma factor:
  • Regulation: repressed by glucose (2.4-fold) (CcpA) PubMed
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant: GP720 (spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References