SspA

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  • Description: small acid-soluble spore protein (major alpha-type SASP)

Gene name sspA
Synonyms
Essential no
Product small acid-soluble spore protein (major alpha-type SASP)
Function protection of spore DNA
MW, pI 6 kDa, 4.731
Gene length, protein length 207 bp, 69 aa
Immediate neighbours ytcI, thiI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SspA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SspA expression.png
























Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

  • Locus tag: BSU29570

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: alpha/beta-type SASP family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2BZ0 (82% identity, 89% similarity)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ralf Moeller, Peter Setlow, Günther Reitz, Wayne L Nicholson
Roles of small, acid-soluble spore proteins and core water content in survival of Bacillus subtilis spores exposed to environmental solar UV radiation.
Appl Environ Microbiol: 2009, 75(16);5202-8
[PubMed:19542328] [WorldCat.org] [DOI] (I p)

Barbara Setlow, Swaroopa Atluri, Ryan Kitchel, Kasia Koziol-Dube, Peter Setlow
Role of dipicolinic acid in resistance and stability of spores of Bacillus subtilis with or without DNA-protective alpha/beta-type small acid-soluble proteins.
J Bacteriol: 2006, 188(11);3740-7
[PubMed:16707666] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Thierry Douki, Barbara Setlow, Peter Setlow
Effects of the binding of alpha/beta-type small, acid-soluble spore proteins on the photochemistry of DNA in spores of Bacillus subtilis and in vitro.
Photochem Photobiol: 2005, 81(1);163-9
[PubMed:15458366] [WorldCat.org] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

B Setlow, K A McGinnis, K Ragkousi, P Setlow
Effects of major spore-specific DNA binding proteins on Bacillus subtilis sporulation and spore properties.
J Bacteriol: 2000, 182(24);6906-12
[PubMed:11092849] [WorldCat.org] [DOI] (P p)

I Bagyan, J Hobot, S Cutting
A compartmentalized regulator of developmental gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(15);4500-7
[PubMed:8755877] [WorldCat.org] [DOI] (P p)

B Setlow, P Setlow
Binding to DNA protects alpha/beta-type, small, acid-soluble spore proteins of Bacillus and Clostridium species against digestion by their specific protease as well as by other proteases.
J Bacteriol: 1995, 177(14);4149-51
[PubMed:7608092] [WorldCat.org] [DOI] (P p)

J M Mason, P Fajardo-Cavazos, P Setlow
Levels of mRNAs which code for small, acid-soluble spore proteins and their LacZ gene fusions in sporulating cells of Bacillus subtilis.
Nucleic Acids Res: 1988, 16(14A);6567-83
[PubMed:2456528] [WorldCat.org] [DOI] (P p)

M J Connors, J M Mason, P Setlow
Cloning and nucleotide sequencing of genes for three small, acid-soluble proteins from Bacillus subtilis spores.
J Bacteriol: 1986, 166(2);417-25
[PubMed:3009398] [WorldCat.org] [DOI] (P p)