Difference between revisions of "SsbB"

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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
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** binds and protects internalized foreign ssDNA {{PubMed|22373918}}
  
 
* '''Protein family:'''
 
* '''Protein family:'''
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* '''[[Localization]]:'''  
 
* '''[[Localization]]:'''  
 
** localizes to cell poles in competent cells [http://www.ncbi.nlm.nih.gov/pubmed/16009133 PubMed]
 
** localizes to cell poles in competent cells [http://www.ncbi.nlm.nih.gov/pubmed/16009133 PubMed]
 +
** localizes to the DNA entry pole during transformation {{PubMed|22373918}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 17:54, 7 March 2012

  • Description: single-strand DNA-binding protein

Gene name ssbB
Synonyms ywpH
Essential no
Product single-strand DNA-binding protein
Function required for efficient genetic transformation
Interactions involving this protein in SubtInteract: SsbB
MW, pI 12 kDa, 6.876
Gene length, protein length 339 bp, 113 aa
Immediate neighbours glcR, ywpG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwpH context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

DNA replication, DNA repair/ recombination

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

  • Locus tag: BSU36310

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • binds and protects internalized foreign ssDNA PubMed
  • Protein family:
  • Paralogous protein(s): SsbA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on Tyr by PtkA, dephosphorylated by PtpZ PubMed
  • Cofactor(s):
  • Effectors of protein activity: phosphorylation strongly increases DNA-binding activity PubMed
  • Localization:
    • localizes to cell poles in competent cells PubMed
    • localizes to the DNA entry pole during transformation PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed at the onset of stationary phase PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References