srfAC

168

surfactin synthetase / competence

locus: BSU_03510

Molecular weight: 143.59 kDa

pI: 4.97

Protein length: 1275 aa

Gene length: 3828 bp

function: antibiotic synthesis

product: surfactin synthetase / competence

essential: no

synonyms: srfAC, comL

List of homologs in different organisms, belongs to COG3319 (Galperin et al., 2021)

Gene

Coordinates: 398,532  402,359

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The protein

Protein family

• [wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)

[wiki|Domains]

• Carrier domain (aa 968-1043) (according to UniProt)

Structure

• [PDB|2VSQ] [pubmed|18583577]
• [AF|Q08787]

Modification

• phosphorylation on Ser-1003 [Pubmed|17218307]

[wiki|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|81845F44CE2C601555066E31E87384FF5D7B4139|srfAA]-[gene|3C484FDB3A440269E87D6B6E8500A875AC456666|srfAB]-[gene|4D76F07D6124D56C7CEBBDAB9E84B2718BF1E7FC|comS]-[gene|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|srfAC]-[gene|9D0914FC38FFF4028C2BA5970DC87B3A1027E253|srfAD]

  description: [Pubmed|1715856]

  regulation

  • expressed at high cell density ([protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]) [Pubmed|16091051]
  • heterogeneous expression, percentage of expressing cells increases during colonization of plant roots [pubmed|34557426]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|25666134], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|8830686], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|comA]: activation, [Pubmed|1715856,16091051], in [regulon|protein:7832489F11F606BCF637EC23BAAB41AD10237EBB|comA regulon]
  • [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]: activation, [Pubmed|16166527], in [regulon|protein:00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR regulon]
  • [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: repression, [Pubmed|12642660], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
  • [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh]: repression, [Pubmed|20817675], in [regulon|protein:5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1715856], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between '[protein|D9A187961CFB9496A6712E9E48AAD384357A3E1C|hxlR]' and '[protein|81845F44CE2C601555066E31E87384FF5D7B4139|srfAA]' [PubMed|20525796]

Biological materials

Mutant

• BKE03510 ([gene|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|srfAC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGTTTCGCTCCCGCCT,  downstream forward: _UP4_TAAATGAAGTGATGAAAGGA
• BKK03510 ([gene|1D5F227860A321506A1AB4BAE63CAD3A66E43BFC|srfAC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03510 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGTTTCGCTCCCGCCT,  downstream forward: _UP4_TAAATGAAGTGATGAAAGGA

References

Reviews

• 20735481,36833349

Research papers

• 18583577,18763711