Difference between revisions of "SpsL"

From SubtiWiki
Jump to: navigation, search
Line 42: Line 42:
  
 
= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[GerE regulon]]}},
 
{{SubtiWiki regulon|[[SigK regulon]]}}
 
{{SubtiWiki regulon|[[SigK regulon]]}}
  
Line 60: Line 61:
  
 
=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
Line 111: Line 109:
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** expressed late during sporulation in the mother cell ([[SigK]]) {{PubMed|15383836}}  
+
** expressed late during sporulation in the mother cell ([[SigK]], [[GerE]]) {{PubMed|25239894,15383836}}  
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[GerE]]: transcription activation {{PubMed|25239894,15383836}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
Line 136: Line 135:
  
 
=References=
 
=References=
<pubmed>9353933 15383836 22960854 </pubmed>
+
<pubmed>9353933 15383836 22960854 25239894</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:03, 23 September 2014

  • Description: dTDP -4-dehydrorhamnose-3,5-epimerase, spore coat polysaccharide synthesis

Gene name spsL
Synonyms ipa-73d
Essential no
Product dTDP -4-dehydrorhamnose-3,5-epimerase
Function rhamnose biosynthesis, spore coat polysaccharide synthesis
Gene expression levels in SubtiExpress: spsL
MW, pI 17 kDa, 4.878
Gene length, protein length 453 bp, 151 aa
Immediate neighbours sivA, spsK
Sequences Protein DNA DNA_with_flanks
Genetic context
SpsL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpsL expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, sporulation proteins

This gene is a member of the following regulons

GerE regulon, SigK regulon

The gene

Basic information

  • Locus tag: BSU37810

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3RYK (dTDP-4-dehydrorhamnose 3,5-epimerase (rfbC) from Bacillus anthracis str. Ames with TDP and PPi bound, 32% identity, 56% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK, GerE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Giuseppina Cangiano, Teja Sirec, Cristina Panarella, Rachele Isticato, Loredana Baccigalupi, Maurilio De Felice, Ezio Ricca
The sps Gene Products Affect the Germination, Hydrophobicity, and Protein Adsorption of Bacillus subtilis Spores.
Appl Environ Microbiol: 2014, 80(23);7293-302
[PubMed:25239894] [WorldCat.org] [DOI] (I p)

Germán Plata, Tobias Fuhrer, Tzu-Lin Hsiao, Uwe Sauer, Dennis Vitkup
Global probabilistic annotation of metabolic networks enables enzyme discovery.
Nat Chem Biol: 2012, 8(10);848-54
[PubMed:22960854] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)