Difference between revisions of "SpsI"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spsJ]]'', ''[[spsG]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spsJ]]'', ''[[spsG]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37840 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37840 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:spsI_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:spsI_context.gif]]

Revision as of 11:34, 14 May 2013

  • Description: glucose-1-phosphate thymidylyltransferase, spore coat polysaccharide synthesis

Gene name spsI
Synonyms ipa-71d
Essential no
Product glucose-1-phosphate thymidylyltransferase
Function rhamnose biosynthesis, spore coat polysaccharide synthesis
Gene expression levels in SubtiExpress: spsI
MW, pI 27 kDa, 5.385
Gene length, protein length 738 bp, 246 aa
Immediate neighbours spsJ, spsG
Sequences Protein DNA DNA_with_flanks
Genetic context
SpsI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpsI expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, sporulation proteins

This gene is a member of the following regulons

SigK regulon

The gene

Basic information

  • Locus tag: BSU37840

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • alpha-D-glucose-1-phosphate + dTTP --> dTDP-glucose PubMed
  • Protein family: glucose-1-phosphate thymidylyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the mother cell (SigK) PubMed
  • Regulatory mechanism:
  • Additional information:
    • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Germán Plata, Tobias Fuhrer, Tzu-Lin Hsiao, Uwe Sauer, Dennis Vitkup
Global probabilistic annotation of metabolic networks enables enzyme discovery.
Nat Chem Biol: 2012, 8(10);848-54
[PubMed:22960854] [WorldCat.org] [DOI] (I p)

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)