spoVS

168

required for dehydratation of the spore core and assembly of the coat, mutation increases [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]-dependent gene expression, might act via [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]

locus: BSU_16980

Molecular weight: 8.66 kDa

pI: 6.47

Protein length: 86 aa

Gene length: 261 bp

function: spore coat assembly, spore core dehydratation

product: unknown

essential: no

synonyms: spoVS

List of homologs in different organisms, belongs to COG2359 (Galperin et al., 2021)

Gene

Coordinates: 1,769,935  1,770,195

Phenotypes of a mutant

• increased [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]-dependent gene expression, this might occur via [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] [Pubmed|16428420]

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The protein

Structure

• [PDB|2EK0] (from ''Thermus thermophilus'', 56% identity)
• [AF|P45693]

Biological materials

Mutant

• GP1601 ([gene|384D4D4C58FCBEB46B762E01478F4081FC6FAE4A|spoVS]::cat), available in [wiki|Jörg Stülke]'s lab
• GP1848 ([gene|154862E3FFA7CEBA5725BAD08E09D0E1C6B7F946|ymdB]-[gene|384D4D4C58FCBEB46B762E01478F4081FC6FAE4A|spoVS]::''spc'' cassette), available in [wiki|Jörg Stülke]'s lab
• BKE16980 ([gene|384D4D4C58FCBEB46B762E01478F4081FC6FAE4A|spoVS]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCTGCTCCCCCTTAGT,  downstream forward: _UP4_TAAAAACAAATAAAGCATTC
• BKK16980 ([gene|384D4D4C58FCBEB46B762E01478F4081FC6FAE4A|spoVS]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16980 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCTGCTCCCCCTTAGT,  downstream forward: _UP4_TAAAAACAAATAAAGCATTC

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

References

• 7559352,18562273,16428420,20525796,35638784