spoVG

168

RNA-binding regulatory protein, negative effector of asymetric septation at the onset of sporulation

locus: BSU_00490

Molecular weight: 10.75 kDa

pI: 5.11

Protein length: 97 aa

Gene length: 294 bp

function: cell division, control of sporulation initiation

product: negative effector of asymetric septation

essential: no

synonyms: spoVG

List of homologs in different organisms, belongs to COG2088 (Galperin et al., 2021)

Gene

Coordinates: 55,866 → 56,159

Advertisement

The protein

Protein family

• SpoVG family (single member, according to UniProt)

Structure

• [PDB|2IA9]
• [AF|P28015]

Modification

• phosphorylation on (Ser-66 OR Ser-67 OR Thr-68) [Pubmed|17218307]

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

Expression and Regulation

Operons

• genes: [gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]

  description: [Pubmed|408013]

  regulation

  • repressed by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] [Pubmed|16430695]

  regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: repression, [Pubmed|16430695], in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|2437099], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

  sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|408013], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]

Biological materials

Mutant

• MGNA-A082 (spoVG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/82 NBRP B. subtilis, Japan]
• GP2109 (''[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]''::''tet'', available in [wiki|Jörg Stülke]'s lab)
• BKE00490 (Δ[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE00490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGTAGTTCACCACCTTT,  downstream forward: _UP4_TAAAAAATAACCAAAAAGCA
• BKK00490 (Δ[gene|E9FF83FA90DCD7ECABFD67E95ECFAD3518DDC775|spoVG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK00490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAGTAGTTCACCACCTTT,  downstream forward: _UP4_TAAAAAATAACCAAAAAGCA

Expression vectors

• pGP2163: expression of SpoVG-Strep by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
• pGP2310: expression of SpoVG-Strep by [wiki|pGP382] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 33690958,35638784

Original Publications

• 408013,1850083,16430695,6405278,6411929,10348850,7559352,8419299,3100810,2437099,17218307,20803133,27048798,34102998