spoVFB

168

dipicolinate synthase (subunit B)

locus: BSU_16740

Molecular weight: 21.72 kDa

pI: 6.15

Protein length: 200 aa

Gene length: 603 bp

function: dipicolic acid production

product: dipicolinate synthase (subunit B)

essential: no

synonyms: spoVFB, dpaB

List of homologs in different organisms, belongs to COG5843 (Galperin et al., 2021)

Gene

Coordinates: 1,745,263  1,745,865

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The protein

Catalyzed reaction/ biological activity

• (S)-2,3-dihydrodipicolinate + NADP⁺ --> dipicolinate + H⁺ + NADPH (according to UniProt)

Structure

• [PDB|3MCU] (from B. cereus, 72% identity)
• [AF|Q04810]

Biological materials

Mutant

• BKE16740 ([gene|4D93A13DB9DC55A49525BFF4FD589F4FF67D50E8|spoVFB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTTCCTTTTAATGACGACA,  downstream forward: _UP4_TAAATTTACAGAAAATCTTT
• BKK16740 ([gene|4D93A13DB9DC55A49525BFF4FD589F4FF67D50E8|spoVFB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TTTTCCTTTTAATGACGACA,  downstream forward: _UP4_TAAATTTACAGAAAATCTTT

References

• 8345520,8098035,15699190,35638784