SpoVC

• Description: general stress protein, peptidyl-tRNA hydrolase
  
  

Gene name	spoVC
Synonyms	pth
Essential	yes PubMed
Product	peptidyl-tRNA hydrolase
Function	spore coat formation
MW, pI	20 kDa, 5.58
Gene length, protein length	564 bp, 188 aa
Immediate neighbours	ctc, subA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00530

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: N-substituted aminoacyl-tRNA + H₂O = N-substituted amino acid + tRNA (according to Swiss-Prot)

• Protein family: PTH family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P37470

• KEGG entry: [2]

• E.C. number: 3.1.1.29

Expression and regulation

• Operon:

• Sigma factor: SigB PubMed

• Regulation: induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jeanne Menez, Richard H Buckingham, Miklos de Zamaroczy, Celine Karmazyn Campelli
Peptidyl-tRNA hydrolase in Bacillus subtilis, encoded by spoVC, is essential to vegetative growth, whereas the homologous enzyme in Saccharomyces cerevisiae is dispensable.
Mol Microbiol: 2002, 45(1);123-9
[PubMed:12100553] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)