spoVAD
168
essential for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2 ) into developing spores and DPA release during [category|SW.4.2.4|Germination], likely germination protein
locus
BSU_23410
Molecular weight
35.84 kDa
pI
5.24
function
likely germination protein
product
binding protein for DPA
essential
no
synonyms
spoVAD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5837 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,440,775 2,441,791
The protein
Catalyzed reaction/ biological activity
maybe involved in spore [wiki|germination] [Pubmed|22327596]
binding of both DPA(2,6) and Ca(2 )-DPA(2,6) [Pubmed|22328679]
forms a membrane complex with [protein|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC] and [protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] in vitro [pubmed|35654455]
functions like a "plug" for the [protein|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC]/[protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] membrane channel [pubmed|35654455]
Structure
[PDB|3LM6]
[AF|P40869]
[wiki|Localization]
cytoplasmic side of the spore inner membrane - that depends on [protein|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC]/[protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] [Pubmed|35654455,23335419,16077113]
Additional information
6,500 molecules are present per spore [Pubmed|23749970]
Expression and Regulation
additional information
6,500 molecules are present per spore [PubMed|23749970]
Biological materials
Mutant
BKE23410 ([gene|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT, downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC
BKK23410 ([gene|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT, downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC
labs
[wiki|Peter Setlow], University of Connecticut Health Center, USA
References
Reviews
Original Publications
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Time of last update: 2024-04-24 10:02:39
Author of last update: Jstuelk