spoVAD

168

essential for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2 ) into developing spores and DPA release during [category|SW.4.2.4|Germination], likely germination protein

locus: BSU_23410

Molecular weight: 35.84 kDa

pI: 5.24

Protein length: 338 aa

Gene length: 1017 bp

function: likely germination protein

product: binding protein for DPA

essential: no

synonyms: spoVAD

List of homologs in different organisms, belongs to COG5837 (Galperin et al., 2021)

Gene

Coordinates: 2,440,775  2,441,791

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The protein

Catalyzed reaction/ biological activity

• maybe involved in spore [wiki|germination] [Pubmed|22327596]
• binding of both DPA(2,6) and Ca(2 )-DPA(2,6) [Pubmed|22328679]
• forms a membrane complex with [protein|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC] and [protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] in vitro [pubmed|35654455]
• functions like a "plug" for the [protein|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC]/[protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] membrane channel [pubmed|35654455]

Structure

• [PDB|3LM6]
• [AF|P40869]

[wiki|Localization]

• cytoplasmic side of the spore inner membrane - that depends on [protein|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC]/[protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] [Pubmed|35654455,23335419,16077113]

Additional information

• 6,500 molecules are present per spore [Pubmed|23749970]

Expression and Regulation

additional information

• 6,500 molecules are present per spore [PubMed|23749970]

Biological materials

Mutant

• BKE23410 ([gene|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT,  downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC
• BKK23410 ([gene|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23410 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_TGTTAATTTCATTTTCTTCT,  downstream forward: _UP4_GAGCGTGCAGGAGGTGCATC

labs

• [wiki|Peter Setlow], University of Connecticut Health Center, USA

References

Reviews

• 35638784

Original Publications

• 17573930,3114420,16077113,11751839,15451103,3926949,17158659,6432957,22328679,7934830,15699190,1903432,8755877,22327596,22328679,22493018,23335419,23746146,22343299,23749970,24752279,26731423,35654455