spoVAC

168

required for the uptake of the 1:1 chelate of pyridine-2,6-dicarboxylic acid (DPA(2,6)) and Ca(2 ) into developing spores and release during [category|SW.4.2.4|Germination], required for spore maturation

locus: BSU_23420

Molecular weight: 15.97 kDa

pI: 9.46

Protein length: 150 aa

Gene length: 453 bp

function: spore maturation

product: subunit of dipicolinate uptake complex

essential: no

synonyms: spoVAC

List of homologs in different organisms, belongs to COG5836 (Galperin et al., 2021)

Gene

Coordinates: 2,441,804 → 2,442,256

Advertisement

The protein

Catalyzed reaction/ biological activity

• forms a membrane channel with [protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] [pubmed|35654455]
• forms a membrane complex with [protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] and [protein|9531F0A0DC4AE5696FFBAC9C2A773F15840E3255|spoVAD] in vitro [pubmed|35654455]

Structure

• [AF|P40868]

[wiki|Localization]

• inner spore membrane [Pubmed|26731423,35654455]

Additional information

• stability in spores depends on [protein|31D6CB7EC20C39C1815CA4552B8B9C4BE304E86B|spoVAEB] [pubmed|35654455]

Biological materials

Mutant

• BKE23420 (Δ[gene|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTGAAACCTCCTTATG,  downstream forward: _UP4_CTGATGTCATAGGAGGAGAA
• BKK23420 (Δ[gene|B280951A232E941FB0A6C8834049765BE1E16437|spoVAC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23420 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTTGAAACCTCCTTATG,  downstream forward: _UP4_CTGATGTCATAGGAGGAGAA

labs

• [wiki|Peter Setlow], University of Connecticut Health Center, USA

References

Reviews

• 35638784

Original Publications

• 3114420,11751839,15451103,3926949,17158659,6432957,7934830,15699190,1903432,8755877,22328679,21398556,24666282,24752279,26731423,35654455