spoIVFB

168

intramembrane metalloprotease (site 2 protease), processing of pro-sigma-K to active [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]

locus: BSU_27970

Molecular weight: 33.49 kDa

pI: 8.48

Protein length: 288 aa

Gene length: 867 bp

function: processing of pro-sigma-K to active [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]

product: intramembrane metalloprotease

essential: no

synonyms: spoIVFB

List of homologs in different organisms

Gene

Coordinates: 2,855,973  2,856,839

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The protein

Catalyzed reaction/ biological activity

• processing of pro-sigma-K to active [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK] [Pubmed|19805276]

Protein family

• [wiki|peptidase M50B family] (according to UniProt)

[wiki|Domains]

• C-terminal [wiki|CBS domain], this domain binds ATP [Pubmed|19805276]

[wiki|Cofactors]

• Zn²⁺ [pubmed|35007155]

Structure

• [AF|P26937]

Effectors of protein activity

• ATP regulates substrate access to the active site and renders cleavage sensitive to the cellular energy level [Pubmed|19805276]
• [protein|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB] is kept inactive by the interaction with [protein|AB2A3422277040107AAF90358BBE58608F8E0738|spoIVFA] and [protein|CB5D3A2E5BEE336539BEB2D7C5D9C3A63C78FD01|bofA] [pubmed|35471152,15087499]

[wiki|Localization]

• mother cell integral membrane protein [Pubmed|24243021,11959848]

Biological materials

Mutant

• BKE27970 ([gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA,  downstream forward: _UP4_TAAAACTGATTGACAAACGC
• BKK27970 ([gene|8801B095C2CE36F3E0B9DBBF489FFB217087DC0A|spoIVFB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GATCTTTAAGATAAGGTCGA,  downstream forward: _UP4_TAAAACTGATTGACAAACGC

References

Reviews

• 20836086,23479438,19189971,24099006,31350897,33538382,34410368,35638784

Original Publications

• 11959848,9501233,12940997,1577688,12060714,9078383,1942049,10611287,15383836,16818230,19805276,15699190,23585539,23995631,15087499,24243021,22383849,26953342,29180425,30403663,32515031,35007155,35471152