SpoIIQ

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  • Description: component of the SpoIIIAH-SpoIIQ type III secretion system residing in the forespore membrane, required for anchoring of proteins on both sides of the sporulation septum

Gene name spoIIQ
Synonyms ywnI
Essential no
Product component of a type III secretion system
Function forespore engulfment
MW, pI 30 kDa, 4.475
Gene length, protein length 849 bp, 283 aa
Immediate neighbours ywnJ, ywnH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpoIIQ context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU36550

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: forespore membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jeffrey Meisner, Charles P Moran
A LytM domain dictates the localization of proteins to the mother cell-forespore interface during bacterial endospore formation.
J Bacteriol: 2011, 193(3);591-8
[PubMed:21097616] [WorldCat.org] [DOI] (I p)

Pablo Meyer, Jennifer Gutierrez, Kit Pogliano, Jonathan Dworkin
Cell wall synthesis is necessary for membrane dynamics during sporulation of Bacillus subtilis.
Mol Microbiol: 2010, 76(4);956-70
[PubMed:20444098] [WorldCat.org] [DOI] (I p)

Thierry Doan, Cecile Morlot, Jeffrey Meisner, Monica Serrano, Adriano O Henriques, Charles P Moran, David Z Rudner
Novel secretion apparatus maintains spore integrity and developmental gene expression in Bacillus subtilis.
PLoS Genet: 2009, 5(7);e1000566
[PubMed:19609349] [WorldCat.org] [DOI] (I p)

Jeffrey Meisner, Xin Wang, Monica Serrano, Adriano O Henriques, Charles P Moran
A channel connecting the mother cell and forespore during bacterial endospore formation.
Proc Natl Acad Sci U S A: 2008, 105(39);15100-5
[PubMed:18812514] [WorldCat.org] [DOI] (I p)

Amy H Camp, Richard Losick
A novel pathway of intercellular signalling in Bacillus subtilis involves a protein with similarity to a component of type III secretion channels.
Mol Microbiol: 2008, 69(2);402-17
[PubMed:18485064] [WorldCat.org] [DOI] (I p)

Briana M Burton, Kathleen A Marquis, Nora L Sullivan, Tom A Rapoport, David Z Rudner
The ATPase SpoIIIE transports DNA across fused septal membranes during sporulation in Bacillus subtilis.
Cell: 2007, 131(7);1301-12
[PubMed:18160039] [WorldCat.org] [DOI] (P p)

Nathalie Campo, Kathleen A Marquis, David Z Rudner
SpoIIQ anchors membrane proteins on both sides of the sporulation septum in Bacillus subtilis.
J Biol Chem: 2008, 283(8);4975-82
[PubMed:18077456] [WorldCat.org] [DOI] (P p)

Shinobu Chiba, Kristina Coleman, Kit Pogliano
Impact of membrane fusion and proteolysis on SpoIIQ dynamics and interaction with SpoIIIAH.
J Biol Chem: 2007, 282(4);2576-86
[PubMed:17121846] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Jonathan Dworkin, Richard Losick
Developmental commitment in a bacterium.
Cell: 2005, 121(3);401-9
[PubMed:15882622] [WorldCat.org] [DOI] (P p)

Thierry Doan, Kathleen A Marquis, David Z Rudner
Subcellular localization of a sporulation membrane protein is achieved through a network of interactions along and across the septum.
Mol Microbiol: 2005, 55(6);1767-81
[PubMed:15752199] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Bill Blaylock, Xin Jiang, Aileen Rubio, Charles P Moran, Kit Pogliano
Zipper-like interaction between proteins in adjacent daughter cells mediates protein localization.
Genes Dev: 2004, 18(23);2916-28
[PubMed:15574594] [WorldCat.org] [DOI] (P p)

Aileen Rubio, Kit Pogliano
Septal localization of forespore membrane proteins during engulfment in Bacillus subtilis.
EMBO J: 2004, 23(7);1636-46
[PubMed:15044948] [WorldCat.org] [DOI] (P p)

J A Londoño-Vallejo, C Fréhel, P Stragier
SpoIIQ, a forespore-expressed gene required for engulfment in Bacillus subtilis.
Mol Microbiol: 1997, 24(1);29-39
[PubMed:9140963] [WorldCat.org] [DOI] (P p)