SpoIIQ

• Description: component of the SpoIIIAH-SpoIIQ type III secretion system residing in the forespore membrane, required for anchoring of proteins on both sides of the sporulation septum
  
  

Gene name	spoIIQ
Synonyms	ywnI
Essential	no
Product	part of the transmembrane channel linking the mother cell and the forespore
Function	forespore encasement by the spore coat
Gene expression levels in SubtiExpress: spoIIQ
Interactions involving this protein in SubtInteract: SpoIIQ
MW, pI	30 kDa, 4.475
Gene length, protein length	849 bp, 283 aa
Immediate neighbours	ywnJ, ywnH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU36550

Database entries

• BsubCyc: BSU36550

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for forespore encasement by the spore coat PubMed
  • required for the recruitment of SpoIIIAH to the sporulation septum on the mother cell side PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SpoIIIAH(12)-SpoIIQ(12) (interaction between proteins membrane proteins expressed in the mother cell and the forespore, respectively; the complex forms a 60 Å channel made up of twelve subunits of each protein) PubMed
  • SpoIIQ-SpoIIE

• Localization:
  • membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with SpoIIIAH) PubMed
  • proper localization requires SpoIIIAH and degradation of septal peptidoglycan by the cell wall hydrolases SpoIID and SpoIIP PubMed

Database entries

• BsubCyc: BSU36550

• Structure:
  • 3UZO; 3TUF (the SpoIIQ-SpoIIIAH pore forming complex) PubMed

• UniProt: P71044

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIQ PubMed

• Expression browser: spoIIQ PubMed

• Sigma factor: SigF PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Adam D Crawshaw, Mónica Serrano, Will A Stanley, Adriano O Henriques, Paula S Salgado
A mother cell-to-forespore channel: current understanding and future challenges.
FEMS Microbiol Lett: 2014, 358(2);129-36
[PubMed:25105965] [WorldCat.org] [DOI] (I p)

Lotte Søgaard-Andersen
Stably bridging a great divide: localization of the SpoIIQ landmark protein in Bacillus subtilis.
Mol Microbiol: 2013, 89(6);1019-24
[PubMed:23944268] [WorldCat.org] [DOI] (I p)

Original publications

Jennifer Fredlund, Dan Broder, Tinya Fleming, Clémence Claussin, Kit Pogliano
The SpoIIQ landmark protein has different requirements for septal localization and immobilization.
Mol Microbiol: 2013, 89(6);1053-68
[PubMed:23859254] [WorldCat.org] [DOI] (I p)

Christopher D A Rodrigues, Kathleen A Marquis, Jeffrey Meisner, David Z Rudner
Peptidoglycan hydrolysis is required for assembly and activity of the transenvelope secretion complex during sporulation in Bacillus subtilis.
Mol Microbiol: 2013, 89(6);1039-52
[PubMed:23834622] [WorldCat.org] [DOI] (I p)

Jeffrey Meisner, Tatsuya Maehigashi, Ingemar André, Christine M Dunham, Charles P Moran
Structure of the basal components of a bacterial transporter.
Proc Natl Acad Sci U S A: 2012, 109(14);5446-51
[PubMed:22431613] [WorldCat.org] [DOI] (I p)

Vladimir M Levdikov, Elena V Blagova, Amanda McFeat, Mark J Fogg, Keith S Wilson, Anthony J Wilkinson
Structure of components of an intercellular channel complex in sporulating Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(14);5441-5
[PubMed:22431604] [WorldCat.org] [DOI] (I p)

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Jeffrey Meisner, Charles P Moran
A LytM domain dictates the localization of proteins to the mother cell-forespore interface during bacterial endospore formation.
J Bacteriol: 2011, 193(3);591-8
[PubMed:21097616] [WorldCat.org] [DOI] (I p)

Pablo Meyer, Jennifer Gutierrez, Kit Pogliano, Jonathan Dworkin
Cell wall synthesis is necessary for membrane dynamics during sporulation of Bacillus subtilis.
Mol Microbiol: 2010, 76(4);956-70
[PubMed:20444098] [WorldCat.org] [DOI] (I p)

Thierry Doan, Cecile Morlot, Jeffrey Meisner, Monica Serrano, Adriano O Henriques, Charles P Moran, David Z Rudner
Novel secretion apparatus maintains spore integrity and developmental gene expression in Bacillus subtilis.
PLoS Genet: 2009, 5(7);e1000566
[PubMed:19609349] [WorldCat.org] [DOI] (I p)

Jeffrey Meisner, Xin Wang, Monica Serrano, Adriano O Henriques, Charles P Moran
A channel connecting the mother cell and forespore during bacterial endospore formation.
Proc Natl Acad Sci U S A: 2008, 105(39);15100-5
[PubMed:18812514] [WorldCat.org] [DOI] (I p)

Amy H Camp, Richard Losick
A novel pathway of intercellular signalling in Bacillus subtilis involves a protein with similarity to a component of type III secretion channels.
Mol Microbiol: 2008, 69(2);402-17
[PubMed:18485064] [WorldCat.org] [DOI] (I p)

Briana M Burton, Kathleen A Marquis, Nora L Sullivan, Tom A Rapoport, David Z Rudner
The ATPase SpoIIIE transports DNA across fused septal membranes during sporulation in Bacillus subtilis.
Cell: 2007, 131(7);1301-12
[PubMed:18160039] [WorldCat.org] [DOI] (P p)

Nathalie Campo, Kathleen A Marquis, David Z Rudner
SpoIIQ anchors membrane proteins on both sides of the sporulation septum in Bacillus subtilis.
J Biol Chem: 2008, 283(8);4975-82
[PubMed:18077456] [WorldCat.org] [DOI] (P p)

Shinobu Chiba, Kristina Coleman, Kit Pogliano
Impact of membrane fusion and proteolysis on SpoIIQ dynamics and interaction with SpoIIIAH.
J Biol Chem: 2007, 282(4);2576-86
[PubMed:17121846] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Jonathan Dworkin, Richard Losick
Developmental commitment in a bacterium.
Cell: 2005, 121(3);401-9
[PubMed:15882622] [WorldCat.org] [DOI] (P p)

Thierry Doan, Kathleen A Marquis, David Z Rudner
Subcellular localization of a sporulation membrane protein is achieved through a network of interactions along and across the septum.
Mol Microbiol: 2005, 55(6);1767-81
[PubMed:15752199] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Bill Blaylock, Xin Jiang, Aileen Rubio, Charles P Moran, Kit Pogliano
Zipper-like interaction between proteins in adjacent daughter cells mediates protein localization.
Genes Dev: 2004, 18(23);2916-28
[PubMed:15574594] [WorldCat.org] [DOI] (P p)

Aileen Rubio, Kit Pogliano
Septal localization of forespore membrane proteins during engulfment in Bacillus subtilis.
EMBO J: 2004, 23(7);1636-46
[PubMed:15044948] [WorldCat.org] [DOI] (P p)

J A Londoño-Vallejo, C Fréhel, P Stragier
SpoIIQ, a forespore-expressed gene required for engulfment in Bacillus subtilis.
Mol Microbiol: 1997, 24(1);29-39
[PubMed:9140963] [WorldCat.org] [DOI] (P p)