Difference between revisions of "SpoIIIAH"

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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
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|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || component of a type III secretion system
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|style="background:#ABCDEF;" align="center"| '''Product''' || part of the transmembrane channel linking the mother cell and the forespore
 
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|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || activation of [[SigG]]
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|style="background:#ABCDEF;" align="center"|'''Function''' || activation of [[SigG]], forespore encasement by the spore coat
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SpoIIIAH SpoIIIAH]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SpoIIIAH SpoIIIAH]
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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block of sporulation after engulfment
+
* block of sporulation after engulfment
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
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−
 
  
  
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
 +
** required for forespore encasement by the spore coat {{PubMed|22171814}}
  
 
* '''Protein family:'''
 
* '''Protein family:'''
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* '''[[Localization]]:'''  
 
* '''[[Localization]]:'''  
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** membrane protein [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
+
** membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with [[SpoIIQ]]) {{PubMed|22431604,22431613,22171814,18485064}}
  
 
=== Database entries ===
 
=== Database entries ===
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=References=
 
=References=
 
'''Additional publications:'''  {{PubMed|22431604}}
 
'''Additional publications:'''  {{PubMed|22431604}}
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<pubmed>15752199,18485064,15574594,18812514,1766372,17693505,19609349 , 17121846 21097616 22431613</pubmed>
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<pubmed>15752199,18485064,15574594,18812514,1766372,17693505,19609349 , 17121846 21097616 22431613 22171814</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:59, 29 July 2012

  • Description: component of the SpoIIIA-SpoIIQ type III secretion system residing in the forespore membrane, required for SigG activation

Gene name spoIIIAH
Synonyms
Essential no
Product part of the transmembrane channel linking the mother cell and the forespore
Function activation of SigG, forespore encasement by the spore coat
Interactions involving this protein in SubtInteract: SpoIIIAH
MW, pI 23 kDa, 4.627
Gene length, protein length 654 bp, 218 aa
Immediate neighbours accB, spoIIIAG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpoIIIAH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpoIIIAH expression.png

















Categories containing this gene/protein

protein secretion, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

The gene

Basic information

  • Locus tag: BSU24360

Phenotypes of a mutant

  • block of sporulation after engulfment

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • required for forespore encasement by the spore coat PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
    • SpoIIIAH-SpoIIQ (interaction between proteins membrane proteins expressed in the mother cell and the forespore, respectively; the complex forms a 60 Å channel made up of twelve subunits of each protein) PubMed
  • Localization:
    • membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with SpoIIQ) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information: the internal promoter is essential for sporulation, it is twice as active as the promoter in front of spoIIIAA, suggesting that SpoIIIAG and SpoIIIAH are required in larger amounts as compared to the other products of the operon PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

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