spoIIIAH

spoIIIAH
168

component of the [protein|search|SpoIIIA]-[protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] type III secretion system residing in the forespore membrane, required for [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG] activation

locus
BSU_24360
Molecular weight
23.58 kDa
pI
4.63
Protein length
Gene length
function
activation of [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG], forespore encasement by the spore coat
product
part of the transmembrane channel linking the mother cell and the forespore
essential
no
synonyms
spoIIIAH

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5829 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,532,353  2,533,009
Phenotypes of a mutant
reduced sporulation efficiency (1 to 10% compared to wild type) [Pubmed|26735940]
the ''[gene|8F3751A3D0910C9BED7FBCE90AFF7FDACFDCBC0B|spoIIIL] [gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]'' double mutant has a severe sporulation defect (0.001%) [Pubmed|26735940]
block of sporulation after engulfment
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The protein
Catalyzed reaction/ biological activity
required for forespore encasement by the spore coat [Pubmed|22171814]
[wiki|Domains]
contains a ring-building motif (RBM) domain [pubmed|34808342]
Structure
[PDB|3UZO]; [PDB|3TUF] (the [protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ]-[protein|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH] pore forming complex)  [Pubmed|22431604,22431613]
[AF|P49785]
[wiki|Localization]
membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with [protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ]) [Pubmed|22431604,22431613,22171814,18485064]
proper recruitment to the sporulation septum on the mother cell side requires [protein|3282D2C25468776881778006F182FCF322C4821D|spoIIQ] [Pubmed|23834622]
Expression and Regulation
Operons
genes
[gene|70408C17CF8681998F7FD933D4BFF71D052626D9|yqhV]-[gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]-[gene|D20198968665D3726839B2DC7F0638614319B853|spoIIIAB]-[gene|5CDBE6E299C7E6606D3C52E509CFED6F41F1E3F8|spoIIIAC]-[gene|576E36DA79F1ED097D649C35C5B59622FF1CFEF3|spoIIIAD]-[gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]-[gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]-[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
description
[Pubmed|1766372]
regulation
both promoters: expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|18485064], switched off by [wiki|SpoIIID] [Pubmed|15383836]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,18485064], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Open in new tab

[gene|70408C17CF8681998F7FD933D4BFF71D052626D9|yqhV]→[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]

2024-01-22 02:03:35

Jstuelk

53

2962C722B0487E202C1BFF79DD1ECDECA2A12F2C

1576F70579A1A6BA3390B10D16041D0C1E75C377

genes
[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
description
[Pubmed|17693505]
regulation
both promoters: expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|18485064], switched off by [wiki|SpoIIID] [Pubmed|15383836]
additional information
for a discussion of the relevance of the operon-internal promoter, see [pubmed|35913150]
Open in new tab

[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]→[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]

2024-03-23 11:52:32

Jstuelk

56

1A604B61136A462F82D5CAFEBE0BB4664DB0AEBB

96C8A89F41F89A444EA85216D1474F4BA1714D7E

genes
[gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]-[gene|D20198968665D3726839B2DC7F0638614319B853|spoIIIAB]-[gene|5CDBE6E299C7E6606D3C52E509CFED6F41F1E3F8|spoIIIAC]-[gene|576E36DA79F1ED097D649C35C5B59622FF1CFEF3|spoIIIAD]-[gene|9D4AE9AE191BFA82791549852782DC1B959A6897|spoIIIAE]-[gene|8A8C67AB955660FA41039B45F60A5B9F7B57704E|spoIIIAF]-[gene|5456B0C27EAE89F5B9E750D65E1E3C6EDA27C9FA|spoIIIAG]-[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]
description
[Pubmed|18485064]
regulation
both promoters: expressed during sporulation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|18485064], switched off by [wiki|SpoIIID] [Pubmed|15383836]
Open in new tab

[gene|5FE74F270B28A404BFE0DAA4EB243009EEDEACF0|spoIIIAA]→[gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]

2024-03-25 02:36:02

Jstuelk

62

8E4AE6460669048935F7AFEA330AB034477EDE0F

D37B011018A3858E66098C2BA5678469F573873A

Biological materials
Mutant
BKE24360 ([gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]::erm trpC2)available at [http://bgsc.org/getdetail.php?bgscid=BKE24360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCAAACGGTTTGTTTTTTAA,  downstream forward: _UP4_TAAGAATGAGGGAAAAAAGC
BKK24360 ([gene|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|spoIIIAH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCAAACGGTTTGTTTTTTAA,  downstream forward: _UP4_TAAGAATGAGGGAAAAAAGC
labs
[wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
References
Reviews
23944268,25105965,31350897,32660383,33651833,35638784,35913150
Original Publications
15752199,18485064,15574594,18812514,1766372,17693505,19609349,17121846,21097616,22431613,22171814,23834622,22431604,23859254,25356555,26735940,27381174,27681621,33383000,33523946,34808342

00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC

Page visits: 3576

Time of last update: 2024-03-28 18:18:25

Author of last update: Jstuelk