spo0A

spo0A
168

[wiki|phosphorelay] regulator, initiation of [wiki|sporulation], coordinates [wiki|DNA replication] and initiation of [wiki|sporulation] by binding to sites close to the oriC

locus
BSU_24220
Molecular weight
29.54 kDa
pI
5.99
Protein length
Gene length
function
initiation of [wiki|sporulation]
product
[wiki|phosphorelay] response regulator
essential
no
synonyms
spo0A, spo0C, spo0G, spoIIL, sof-1

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG5801 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,518,023  2,518,826
Phenotypes of a mutant
inactivation of ''[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]'' restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]
altered cell death pattern in colonies [Pubmed|23012477]
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The protein
Catalyzed reaction/ biological activity
activation of gene expression at the onset of [wiki|sporulation] ([wiki|Spo0A regulon])
coordination between [wiki|DNA replication] and initiation of [wiki|sporulation] by binding to sites close to the ''oriC'' (this limits re-initiation of replication) [Pubmed|23301687]
required for [wiki|biofilm formation] [Pubmed|11886552]
required for [wiki|sliding] [Pubmed|26152584]
[wiki|Domains]
[wiki|Response regulatory domain] (aa 5-123) (according to UniProt)
Structure
[PDB|1QMP] (with phosphorylated aspartate, ''Geobacillus stearothermophilus''),  [PDB|1FC3] (trans-activation domain, ''Geobacillus stearothermophilus''),  [PDB|1LQ1] (complex with DNA)
[AF|P06534]
Modification
receives phosphorylation from [protein|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B], dephosphorylation by [protein|A574974B6F4FF46DC69E03AE021651C67089866F|spo0E], direct phosphorylation by [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|kinC] upon potassium leakage [Pubmed|19114652]
phosphorylation is enhanced in a [gene|7CD46C64BE69EDADF58B634DC50C3F61BFFEC5B5|rho] mutant [pubmed|28723971]
Effectors of protein activity
the phosphorylation state affects DNA binding activity
interaction with [protein|331993A875907C10C77105FD8DDD86D4412CE405|mecA] inhibits the transcriptional activity of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]P [Pubmed|21435029]
transcription activation by [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]-P is inhibited by interaction with [protein|331993A875907C10C77105FD8DDD86D4412CE405|mecA] (in complex with [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|clpC]) [pubmed|29446505]
Expression and Regulation
Operons
genes
[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]
description
[Pubmed|11254139]
regulation
expression increases when drowth rate declines [Pubmed|21552330]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|1391039], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11254139,1569009], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|11254139,1569009], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
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[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]

2024-03-23 14:57:16

ghost

80

939a2b70d86629f76e63ceb64e25ebf369db367c

0A60D35CF5748D0AB46331261B712042D6CCCED2

additional information
efficient translation depends on [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] [pubmed|36651772]
Biological materials
Mutant
MGNA-C448 (spo0A::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2446 NBRP B. subtilis, Japan]
BKG9 (''[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]''::''aphA3''), available in [wiki|Jörg Stülke]'s lab
SS995 (''[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]''::''ery'') [Pubmed|14761993]
BKE24220 ([gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGTTTCTTCCTCCCCAAA,  downstream forward: _UP4_TAAACATGAGCTTATTAAGT
BKK24220 ([gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGTTTCTTCCTCCCCAAA,  downstream forward: _UP4_TAAACATGAGCTTATTAAGT
3NA (spo0A3), available at [http://bgsc.org/getdetail.php?bgscid=1S1 BGSC], [Pubmed|4986704], [Pubmed|25767229], [https://www.ncbi.nlm.nih.gov/nuccore/CP010314 Genome Sequence]
labs
[wiki|Tony Wilkinson], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]
[wiki|Imrich Barak], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]
[wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
References
Reviews
19995980,20030732,20157337,11286862,20833318,20955377,22303284,24914187,26458398,28075389,28886686,31350897,32156829,32156813,35195292,35638784
Original Publications
34565366,14679239,1569009,20413551,20404177,19528067,19581368,8730857,2106683,3157992,11254139,12067336,10869437,16385044,9287005,8127878,9287022,8231806,1905258,16452424,2118505,8207022,12176382,9733708,11069677,12730135,2437099,8288522,16166384,9495766,2118512,26165942,1556084,18296515,7768874,3145384,19114652,20689749,1537790,9658000,8509330,14762002,1846779,2981817,9477965,8600030,18840696,1391039,14976210,19207565,11679073,12270811,11112444,18978066,20154131,14712656,17157871,15060025,9685500,10852876,21552330,22303282,22745669,23301687,23335417,23660663,25225273,25341802,26152584,23012477,23170957,23169620,22412392,21326214,21435029,21478340,21622736,21949067,21980382,22211522,23927765,24123822,27208661,27216630,27215790,27891124,28033323,28723971,28838935,27501195,29446505,30212463,30808982,31481381,31760912,35012345,36372802,36651772,36748575,36786593,37380492,37485800,37756393
The [wiki|Spo0A regulon]
14651647,15687200

2C54FE2ADC82FF414D732018C90649D477A925AD

Page visits: 21947

Time of last update: 2024-03-28 13:17:06

Author of last update: Jstuelk