Difference between revisions of "SplB"
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13930&redirect=T BSU13930] | ||
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/splAB.html] | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/splAB.html] | ||
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=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU13930&redirect=T BSU13930] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4FHC 4FHC] (SplB from ''Geobacillus thermodenitrificans'', 72% identity, 90% similarity) {{PubMed|22761404}} | * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4FHC 4FHC] (SplB from ''Geobacillus thermodenitrificans'', 72% identity, 90% similarity) {{PubMed|22761404}} | ||
Revision as of 13:34, 2 April 2014
- Description: spore photoproduct lyase
| Gene name | splB |
| Synonyms | spl |
| Essential | no |
| Product | spore photoproduct lyase |
| Function | protection of spore DNA against photodamage |
| Gene expression levels in SubtiExpress: splB | |
| MW, pI | 39 kDa, 8.524 |
| Gene length, protein length | 1026 bp, 342 aa |
| Immediate neighbours | splA, ykwB |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
DNA repair/ recombination, sporulation proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13930
Phenotypes of a mutant
Database entries
- BsubCyc: BSU13930
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAM
- Effectors of protein activity:
Database entries
- BsubCyc: BSU13930
- UniProt: P37956
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant: BP130 (del splB::spc), available in Fabian Commichau's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Structure of the spore photoproduct lesion in DNA
Isha Singh, Yajun Jian, Lei Li, Millie M Georgiadis
The structure of an authentic spore photoproduct lesion in DNA suggests a basis for recognition.
Acta Crystallogr D Biol Crystallogr: 2014, 70(Pt 3);752-9
[PubMed:24598744]
[WorldCat.org]
[DOI]
(I p)
Original publications
