SplA

• Description: transcriptional negative regulator of the spore photoproduct lyase operon
  
  

Gene name	splA
Synonyms	ykxI
Essential	no
Product	transcriptional regulator
Function	regulation of the splA-splB operon
MW, pI	9 kDa, 4.419
Gene length, protein length	237 bp, 79 aa
Immediate neighbours	ptsI, splB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU13920

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P37955

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: splA-splB PubMed

• Sigma factor: SigG PubMed

• Regulation: expressed during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:
  • negative autoregulation by SplA PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

M Pedraza-Reyes, F Gutiérrez-Corona, W L Nicholson
Temporal regulation and forespore-specific expression of the spore photoproduct lyase gene by sigma-G RNA polymerase during Bacillus subtilis sporulation.
J Bacteriol: 1994, 176(13);3983-91
[PubMed:8021181] [WorldCat.org] [DOI] (P p)