speD

168

S-adenosylmethionine decarboxylase, required for biofilm formation

locus: BSU_29010

Molecular weight: 13.96 kDa

pI: 4.77

Protein length: 126 aa

Gene length: 381 bp

function: spermidine, polyamine biosynthesis

product: S-adenosylmethionine decarboxylase

essential: no

ec: 4.1.1.50

synonyms: speD, ytcF

List of homologs in different organisms, belongs to COG1586 (Galperin et al., 2021)

Gene

Coordinates: 2,966,413 → 2,966,793

Phenotypes of a mutant

• no [wiki|biofilm formation] [Pubmed|24529384]

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The protein

Catalyzed reaction/ biological activity

• H⁺ + S-adenosyl-L-methionine --> CO₂ + S-adenosyl 3-(methylsulfanyl)propylamine (according to UniProt)

Protein family

• prokaryotic AdoMetDC family (single member, according to UniProt)

Structure

• [PDB|1VR7] (from ''Thermotoga maritima'', 47% identity, 72% similarity)
• [AF|O34426]

Biological materials

Mutant

• MGNA-A122 (ytcF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/122 NBRP B. subtilis, Japan]
• BKE29010 (Δ[gene|CDD5C7B1CE58352D29BB86BCC2C3685D1D8898ED|speD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTCATGGACCCCCCTT,  downstream forward: _UP4_TAAAGTAAATTGCGAACACA
• BKK29010 (Δ[gene|CDD5C7B1CE58352D29BB86BCC2C3685D1D8898ED|speD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29010 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAGTCATGGACCCCCCTT,  downstream forward: _UP4_TAAAGTAAATTGCGAACACA
• GP2599 (Δ[gene|CDD5C7B1CE58352D29BB86BCC2C3685D1D8898ED|speD]::tet comIQ12L) (in DK1042), available in [wiki|Jörg Stülke]'s lab

References

• 10844697,15720552,9723923,21815947,24529384,28546427