Difference between revisions of "SpeA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || spermidine, polyamine biosynthesis
 
|style="background:#ABCDEF;" align="center"|'''Function''' || spermidine, polyamine biosynthesis
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU14630 speA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14630 speA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/cys_meth_and_sulfate_assimilation.html Cys, Met & Sulfate assimilation]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sr1]]'', ''[[yktA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sr1]]'', ''[[yktA]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13336&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14630 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14630 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14630 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:speA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:speA_context.gif]]

Revision as of 12:43, 13 May 2013

Gene name speA
Synonyms cad
Essential no
Product arginine decarboxylase
Function spermidine, polyamine biosynthesis
Gene expression levels in SubtiExpress: speA
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 53 kDa, 5.081
Gene length, protein length 1470 bp, 490 aa
Immediate neighbours sr1, yktA
Sequences Protein DNA Advanced_DNA
Genetic context
SpeA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SpeA expression.png




























Categories containing this gene/protein

miscellaneous metabolic pathways, biofilm formation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14630

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-arginine = agmatine + CO2 (according to Swiss-Prot)
  • Protein family: Orn/Lys/Arg decarboxylase class-I family (according to Swiss-Prot)
  • Paralogous protein(s): YaaO

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Matthew Burrell, Colin C Hanfrey, Ewan J Murray, Nicola R Stanley-Wall, Anthony J Michael
Evolution and multiplicity of arginine decarboxylases in polyamine biosynthesis and essential role in Bacillus subtilis biofilm formation.
J Biol Chem: 2010, 285(50);39224-38
[PubMed:20876533] [WorldCat.org] [DOI] (I p)

Xiao Yan Liu, Jian Lei, Xiang Liu, Xiao Dong Su, Lanfen Li
Preliminary X-ray crystallographic studies of Bacillus subtilis SpeA protein.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2009, 65(Pt 3);282-4
[PubMed:19255484] [WorldCat.org] [DOI] (I p)

A Sekowska, P Bertin, A Danchin
Characterization of polyamine synthesis pathway in Bacillus subtilis 168.
Mol Microbiol: 1998, 29(3);851-8
[PubMed:9723923] [WorldCat.org] [DOI] (P p)