SleB

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  • Description: spore cortex-lytic enzyme

Gene name sleB
Synonyms ypeA
Essential no
Product spore cortex-lytic enzyme
Function degration of the spore cortex, germination
Gene expression levels in SubtiExpress: sleB
MW, pI 33 kDa, 9.506
Gene length, protein length 915 bp, 305 aa
Immediate neighbours ypeB, prsW
Sequences Protein DNA DNA_with_flanks
Genetic context
SleB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SleB expression.png















Categories containing this gene/protein

germination, membrane proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

  • Locus tag: BSU22930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sleB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • contains a signal peptide PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed late during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

B Setlow, J Yu, Y-Q Li, P Setlow
Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite.
Lett Appl Microbiol: 2013, 57(4);259-65
[PubMed:23746146] [WorldCat.org] [DOI] (I p)

Yunfeng Li, Xuan Y Butzin, Andrew Davis, Barbara Setlow, George Korza, Fatma Işik Üstok, Graham Christie, Peter Setlow, Bing Hao
Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.
J Bacteriol: 2013, 195(11);2530-40
[PubMed:23543708] [WorldCat.org] [DOI] (I p)

George Korza, Peter Setlow
Topology and accessibility of germination proteins in the Bacillus subtilis spore inner membrane.
J Bacteriol: 2013, 195(7);1484-91
[PubMed:23335419] [WorldCat.org] [DOI] (I p)

Xuan Yi, Peter Setlow
Studies of the commitment step in the germination of spores of bacillus species.
J Bacteriol: 2010, 192(13);3424-33
[PubMed:20435722] [WorldCat.org] [DOI] (I p)

Atsushi Masayama, Hideyuki Fukuoka, Shiro Kato, Tohru Yoshimura, Michie Moriyama, Ryuichi Moriyama
Subcellular localization of a germiantion-specific cortex-lytic enzyme, SleB, of Bacilli during sporulation.
Genes Genet Syst: 2006, 81(3);163-9
[PubMed:16905870] [WorldCat.org] [DOI] (P p)

Barbara Setlow, Swaroopa Atluri, Ryan Kitchel, Kasia Koziol-Dube, Peter Setlow
Role of dipicolinic acid in resistance and stability of spores of Bacillus subtilis with or without DNA-protective alpha/beta-type small acid-soluble proteins.
J Bacteriol: 2006, 188(11);3740-7
[PubMed:16707666] [WorldCat.org] [DOI] (P p)

R Moriyama, H Fukuoka, S Miyata, S Kudoh, A Hattori, S Kozuka, Y Yasuda, K Tochikubo, S Makino
Expression of a germination-specific amidase, SleB, of Bacilli in the forespore compartment of sporulating cells and its localization on the exterior side of the cortex in dormant spores.
J Bacteriol: 1999, 181(8);2373-8
[PubMed:10197998] [WorldCat.org] [DOI] (P p)