Difference between revisions of "SkfB"
(→This gene is a member of the following regulons) |
(→Expression and regulation) |
||
| Line 105: | Line 105: | ||
** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed] | ** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed] | ||
** repressed during logrithmic growth ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed] | ** repressed during logrithmic growth ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed] | ||
| + | ** induced by phosphate starvation ([[PhoP]]) [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed] | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed] | ** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed] | ||
** [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed] | ** [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed] | ||
| + | ** [[PhoP]]: transcription activation [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed] | ||
* '''Additional information:''' Northern blotting during during phosphate limitation showed an intense 0.25 kb ''[[skfA]]''-specific transcript, and a weaker 6.5 kb ''[[skfA]]-[[skfB]]-[[skfC]]-[[skfE]]-[[skfF]]-[[skfG]]-[[skfH]]'' transcript. The ''[[skfA]]'' transcript is stabilized by a stem-loop structure in the ''[[skfA]]-[[skfB]]'' intergenic region. [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed] | * '''Additional information:''' Northern blotting during during phosphate limitation showed an intense 0.25 kb ''[[skfA]]''-specific transcript, and a weaker 6.5 kb ''[[skfA]]-[[skfB]]-[[skfC]]-[[skfE]]-[[skfF]]-[[skfG]]-[[skfH]]'' transcript. The ''[[skfA]]'' transcript is stabilized by a stem-loop structure in the ''[[skfA]]-[[skfB]]'' intergenic region. [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed] | ||
Revision as of 12:41, 8 November 2011
- Description: synthesis of spore killing factor
| Gene name | skfB |
| Synonyms | ybcP, ybcQ |
| Essential | no |
| Product | unknown |
| Function | synthesis of spore killing factor |
| MW, pI | 45 kDa, 4.934 |
| Gene length, protein length | 1230 bp, 410 aa |
| Immediate neighbours | skfA, skfC |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
Categories containing this gene/protein
toxins, antitoxins and immunity against toxins
This gene is a member of the following regulons
AbrB regulon, PhoP regulon, Spo0A regulon
The gene
Basic information
- Locus tag: BSU01920
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: moaA/nifB/pqqE family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): contains an iron-sulfur cluster
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- UniProt: O31423
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information: Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript. The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
José Eduardo González-Pastor
Cannibalism: a social behavior in sporulating Bacillus subtilis.
FEMS Microbiol Rev: 2011, 35(3);415-24
[PubMed:20955377]
[WorldCat.org]
[DOI]
(I p)
Original Publications
