Difference between revisions of "SkfA"

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(Extended information on the protein)
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* '''Locus tag:''' BSU01910
 
* '''Locus tag:''' BSU01910
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[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=skfA_213941_214108_1 Expression]
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===

Revision as of 12:50, 24 January 2012

  • Description: spore killing factor

Gene name skfA
Synonyms ybcO
Essential no
Product spore killing factor
Function killing of sister cells
MW, pI 5 kDa, 10.648
Gene length, protein length 165 bp, 55 aa
Immediate neighbours ybcM, skfB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbcO context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

toxins, antitoxins and immunity against toxins

This gene is a member of the following regulons

AbrB regulon, PhoP regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU01910

Expression

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (33-fold) PubMed
    • expressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed during logrithmic growth (AbrB) PubMed
    • induced by phosphate starvation (PhoP) PubMed
    • induced by rhamnogalacturonan PubMed
  • Additional information:
    • Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfE-skfF-skfG-skfH transcript.
    • The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed
    • The mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

José Eduardo González-Pastor
Cannibalism: a social behavior in sporulating Bacillus subtilis.
FEMS Microbiol Rev: 2011, 35(3);415-24
[PubMed:20955377] [WorldCat.org] [DOI] (I p)

Original Publications


Additional publications: PubMed