Difference between revisions of "SkfA"

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(Expression and regulation)
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=== Basic information ===
 
=== Basic information ===
  
* '''Coordinates:'''
+
* '''Locus tag:'''
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===

Revision as of 00:48, 3 June 2009

  • Description: spore killing factor

Gene name skfA
Synonyms ybcO
Essential no
Product spore killing factor
Function killing of sister cells
MW, pI 5 kDa, 10.648
Gene length, protein length 165 bp, 55 aa
Immediate neighbours ybcM, skfB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbcO context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: secreted (according to Swiss-Prot)

Database entries

  • Structure:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: repressed by glucose (33-fold) PubMed
  • Regulatory mechanism:
  • Additional information:
    • Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfD-skfE-skfF-skfG-skfH transcript.
    • The skfA transcript is stabilized by a stem-loop structure in the skfA-skfB intergenic region. PubMed
    • The mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed