skfA

skfA
168

spore killing factor

locus
BSU_01910
Molecular weight
5.80 kDa
pI
10.65
Protein length
Gene length
function
killing of sister cells
product
spore killing factor
essential
no
synonyms
skfA, ybcO

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms

This gene is a member of the following regulons

Gene
Coordinates
213,941 → 214,108
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The protein
Structure
[AF|O31422]
Modification
the mature SKF is cyclic 26-amino acid peptide that is posttranslationally modified with one disulfide and one cysteine thioether bridged to the alpha-position of a methionine [Pubmed|20805502], the thioether bond is generated by [protein|F981C605C652D1A4429579A5F24608CE7F570834|skfB] [Pubmed|23282011]
[wiki|Localization]
secreted (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]-[gene|F981C605C652D1A4429579A5F24608CE7F570834|skfB]-[gene|4DFF9924E07D834F37580D73E88451C1671C5111|skfC]-[gene|5886364199844356446C08F08664B301A714E74A|skfE]-[gene|77D93CAEF46EBBB9BAF570714445E194D5F2884E|skfF]-[gene|527AB8EAAF0A1AAD4E5B714B35ED9F78BECC17EC|skfG]-[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH]
description
[Pubmed|16816204]
regulation
expressed under conditions that trigger sporulation ([wiki|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647,15687200 PubMed]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: activation, [PubMed|14651647,15687200], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [PubMed|15687200,17720793], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP]: activation, [Pubmed|16816204], in [regulon|protein:638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|phoP regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16452424], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
Northern blotting during during phosphate limitation showed an intense 0.25 kb '[protein|search|skfA]'-specific transcript, and a weaker 6.5 kb '[protein|search|skfA]-[protein|search|skfB]-[protein|search|skfC]-[protein|search|skfE]-[protein|search|skfF]-[protein|search|skfG]-[protein|search|skfH]' transcript.
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[gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]→[gene|F31B12F80AFC3580FD33811A1C22E74F75DC1A85|skfH]

2024-03-28 01:59:17

ghost

104

f96cb932f6aa46632dc613334e3526e3904b2534

E1A5B1DE7F5A4653FA0FC2E7FE7AAD3454DBFF28

additional information
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
MGNA-B971 (ybcO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1970 NBRP B. subtilis, Japan]
BKE01910 (Δ[gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE01910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAGTAAACCTCCTCTCA,  downstream forward: _UP4_TAACATTTGAGAATAGGGAG
BKK01910 (Δ[gene|DDB0022F50999AC52809D651C9CC5A5FDC71302C|skfA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK01910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATAAGTAAACCTCCTCTCA,  downstream forward: _UP4_TAACATTTGAGAATAGGGAG
GFP fusion
GP1645 ''[gene|154862E3FFA7CEBA5725BAD08E09D0E1C6B7F946|ymdB]''::''spc'' P''skf-gfp'' (''cat'') in the NCIB3610 background, available in [wiki| Jörg Stülke]'s lab
References
Reviews
20955377
Original Publications
34460312,37527273

DDB0022F50999AC52809D651C9CC5A5FDC71302C

Page visits: 5485

Time of last update: 2024-03-28 16:48:04

Author of last update: Jstuelk