SinR

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• Description: transcriptional regulator of post-exponential-phase responses genes <br/><br/>

Gene name	sinR
Synonyms	sin, flaD
Essential	no
Product	transcriptional regulator of post-exponential-phase responses genes
Function	control of biofilm formation
Metabolic function and regulation of this protein in SubtiPathways: <br/>Biofilm, Central C-metabolism
MW, pI	12 kDa, 7.177
Gene length, protein length	333 bp, 111 aa
Immediate neighbours	sinI, tasA
Get the DNA and protein sequences <br/> (Barbe et al., 2009)
Genetic context <br/> <div align="right"> <small>This image was kindly provided by SubtiList</small></div>

<br/><br/><br/><br/><br/><br/>

The gene

Basic information

• Locus tag: BSU24610

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biofilm formation, transcription factor, Spo0A regulon

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repressor of biofilm genes, acts as co-repressor for SlrR PubMed

• Protein family:

• Paralogous protein(s): SlrR

Genes controlled by SinR

• Activation by SinR: comK

• Repression by SinR: aprE, epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO PubMed,kinB, rapG PubMed, sigD, spo0A,spoIIAA-spoIIAB-sigF, spoIIE, spoIIGA-sigE-sigG, spoVG PubMed, yqxM-sipW-tasA PubMed, yvgN PubMed, ywbD PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: SinI and SlrA are antagonists to SinR PubMed, SlrR is also antagonist of SinR PubMed

• Interactions: SlrA-SinR PubMed, SlrR-SinR PubMed, SinR-ScoC, SinR-SinI

• Localization:

Database entries

• Structure: 1B0N (complex SinR-SinI)

• UniProt: P06533

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • sinI-sinR PubMed
  • sinR PubMed

• Sigma factor:
  • sinI: SigA PubMed
  • sinR: SigA PubMed

• Regulation:
  • sinI: repressed under conditions that trigger sporulation (Spo0A) PubMed
  • sinI: repressed during exponential growth (ScoC) PubMed
  • sinI: repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • ScoC: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: TMB079 sinR::spec, GP736 (tetR), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

• FLAG-tag construct: GP960 (spc, based on pGP1331), available in the Stülke lab

• Antibody:

References

Reviews

<pubmed>20395361 20541494 </pubmed>

Original publications

<pubmed>8955328,15661000,8878039,16923912,15104138,16430695,16430696,18047568,18430133,11751836,1906467,11751836,7635837,11751836, 19201793, 10547280, 15104138, 9799632 19788541 19898538 3125149 8932324 20351052 20923420 8422983 </pubmed>