Difference between revisions of "SinR"

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(Biological materials)
(Biological materials)
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** 1S97 (''sinR''::''phleo''), {{PubMed|8422983}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S97&Search=1S97 BGSC]
 
** 1S97 (''sinR''::''phleo''), {{PubMed|8422983}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S97&Search=1S97 BGSC]
 
** GP1672 (''[[sinR]]-[[tasA]]''::''cat'') {{PubMed|24493247}}, available in [[Jörg Stülke]]'s lab
 
** GP1672 (''[[sinR]]-[[tasA]]''::''cat'') {{PubMed|24493247}}, available in [[Jörg Stülke]]'s lab
** GP1663 (''[[yghG]]-[[sinI]]-[[sinR]]-[[tasA]]''), available in [[Jörg Stülke]]'s lab
+
** GP1663 (''[[yqhG]]-[[sinI]]-[[sinR]]-[[tasA]]''), available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 08:07, 21 July 2015

  • Description: transcriptional regulator (Xre family) of post-exponential-phase responses genes

Gene name sinR
Synonyms sin, flaD
Essential no
Product transcriptional regulator (Xre family)

of post-exponential-phase responses genes

Function control of biofilm formation
Gene expression levels in SubtiExpress: sinR
Interactions involving this protein in SubtInteract: SinR
Metabolic function and regulation of this protein in SubtiPathways:
sinR
MW, pI 12 kDa, 7.177
Gene length, protein length 333 bp, 111 aa
Immediate neighbours sinI, tasA
Sequences Protein DNA DNA_with_flanks
Genetic context
SinR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SinR expression.png















Categories containing this gene/protein

transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons

AbrB regulon, ScoC regulon, Spo0A regulon

The SinR regulon

The gene

Basic information

  • Locus tag: BSU24610

Phenotypes of a mutant

  • the mutation suppresses the galactose toxicity to a galE mutant PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Paralogous protein(s): SlrR

Extended information on the protein

  • Kinetic information:
  • Domains:
    • DNA-binding N-terminal domain (aa 1-69) PubMed
    • SinI-binding C-terminal domain (aa 74-111) PubMed
  • Modification:
  • Cofactor(s):

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y (the half-life of the mRNA increases from 3.5 to 13 min) PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 699 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 425 PubMed

Biological materials

  • Expression vector:


  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

PubMed,

Labs working on this gene/protein

Your additional remarks

References

Reviews

Lynne S Cairns, Laura Hobley, Nicola R Stanley-Wall
Biofilm formation by Bacillus subtilis: new insights into regulatory strategies and assembly mechanisms.
Mol Microbiol: 2014, 93(4);587-98
[PubMed:24988880] [WorldCat.org] [DOI] (I p)

Hera Vlamakis, Yunrong Chai, Pascale Beauregard, Richard Losick, Roberto Kolter
Sticking together: building a biofilm the Bacillus subtilis way.
Nat Rev Microbiol: 2013, 11(3);157-68
[PubMed:23353768] [WorldCat.org] [DOI] (I p)

Patrick Piggot
Epigenetic switching: bacteria hedge bets about staying or moving.
Curr Biol: 2010, 20(11);R480-2
[PubMed:20541494] [WorldCat.org] [DOI] (I p)

David Dubnau
Swim or chill: lifestyles of a bacillus.
Genes Dev: 2010, 24(8);735-7
[PubMed:20395361] [WorldCat.org] [DOI] (I p)


Modelling of the SinI/SinR switch

Jennifer S Hallinan, Goksel Misirli, Anil Wipat
Evolutionary computation for the design of a stochastic switch for synthetic genetic circuits.
Annu Int Conf IEEE Eng Med Biol Soc: 2010, 2010;768-74
[PubMed:21095906] [WorldCat.org] [DOI] (P p)

Original publications