Difference between revisions of "SinI"

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(Biological materials)
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** DS91 (spc) NCIB3610 derivate, available in [[Stülke]] lab
 
** DS91 (spc) NCIB3610 derivate, available in [[Stülke]] lab
 
** 1S98 ( ''sinI''::''kan''), {{PubMed|11466285}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S98&Search=1S98 BGSC]
 
** 1S98 ( ''sinI''::''kan''), {{PubMed|11466285}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S98&Search=1S98 BGSC]
 +
** GP1663 (''yghG-sinI-sinR-tasA''), available in [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 13:26, 1 November 2013

  • Description: antagonist of SinR, drives SlrR from the SlrR(LOW) to the SlrR(HIGH) state

Gene name sinI
Synonyms
Essential no
Product antagonist of SinR
Function control of biofilm formation
Gene expression levels in SubtiExpress: sinI
Interactions involving this protein in SubtInteract: SinI
Regulation of this protein in SubtiPathways:
Biofilm
MW, pI 6 kDa, 6.333
Gene length, protein length 171 bp, 57 aa
Immediate neighbours yqhG, sinR
Sequences Protein DNA DNA_with_flanks
Genetic context
SinI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SinI expression.png















Categories containing this gene/protein

transcription factors and their control, transition state regulators, biofilm formation

This gene is a member of the following regulons

AbrB regulon, ScoC regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU24600

Phenotypes of a mutant

  • altered cell death pattern in colonies PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): SlrA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • activated under conditions that trigger sporulation by (Spo0A) interaction PubMed,PubMed
    • repressed during exponential growth (ScoC) PubMed
    • repressed during logrithmic growth (AbrB) PubMed
    • repressed during vegetative growth (SinR) PubMed
  • Additional information:
    • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

  • Mutant:
    • GP959 (spc), available in Stülke lab
    • DS91 (spc) NCIB3610 derivate, available in Stülke lab
    • 1S98 ( sinI::kan), PubMed, available at BGSC
    • GP1663 (yghG-sinI-sinR-tasA), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • FLAG-tag construct (C-term): GP935 (kan), available in Stülke lab
  • Antibody:

Labs working on this gene/ protein

Your additional remarks

References

Reviews

Modelling of the SinI/SinR switch

Original publications