sigX

168

[wiki|RNA polymerase] ECF-type [wiki|sigma factor] SigX, required for resistance against cationic antimicrobial peptides, required for temporary phage resistance

locus: BSU_23100

Molecular weight: 23.03 kDa

pI: 6.09

Protein length: 194 aa

Gene length: 585 bp

function: resistance to cationic antimcrobial peptides

product: [wiki|RNA polymerase] ECF-type [wiki|sigma factor] SigX

essential: no

synonyms: sigX, ypuM

List of homologs in different organisms, belongs to COG1595 (Galperin et al., 2021)

Gene

Coordinates: 2,414,627 → 2,415,211

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The protein

Protein family

• [wiki|Sigma-70 factor family] (according to UniProt)
• [wiki|ECF subfamily] (according to UniProt)

Structure

• [PDB|6DXO] (from Streptomyces venezuelae, 28% identity) [pubmed|29905823]
• [AF|P35165]

[wiki|Localization]

• cell membrane (according to UniProt)

Additional information

• [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX] is activated in non-infected cell following phage infection of neighbor cells [pubmed|34878184]
• Expression of the [wiki|SigX regulon] in increased in ''[gene|7A606B8E952AE8CA4F9A62008BA4B156725BB5B5|ugtP]'' mutants [Pubmed|22362028]

Biological materials

Mutant

• MGNA-A179 (sigX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/179 NBRP B. subtilis, Japan]
• 1A901 ( ''sigX''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A901&Search=1A901 BGSC]
• BKE23100 (Δ[gene|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGAAACCCCTCCGTTC,  downstream forward: _UP4_GAGCTTTTGAGGGAGGAGCT
• BKK23100 (Δ[gene|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23100 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGAAACCCCTCCGTTC,  downstream forward: _UP4_GAGCTTTTGAGGGAGGAGCT

References

Reviews

• 24921931,26901131

Other original publications

• 36815589,11222589,9139908,14993308,12399481,14762009,19047346,14651641,19465659,9636707,16306698,7934830,19767430,9393439,22362028,23103977,20817771,26364265,27137497,27965645,30355672,29905823,31118925,34864869,34878184

The [wiki|SigX regulon]

• 9683469,17675383,10960106