SigX

• Description: RNA polymerase ECF-type sigma factor SigX
  
  

Gene name	sigX
Synonyms	ypuM
Essential	no
Product	RNA polymerase ECF-type sigma factor SigX
Function	cell surface properties
Gene expression levels in SubtiExpress: sigX
Interactions involving this protein in SubtInteract: SigX
MW, pI	23 kDa, 6.086
Gene length, protein length	582 bp, 194 aa
Immediate neighbours	rsiX, resE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigX regulon, YvrHb regulon

The gene

Basic information

• Locus tag: BSU23100

Database entries

• BsubCyc: BSU23100

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ECF subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RsiX-SigX PubMed
  • SigX-RpoB-RpoC

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU23100

• Structure:

• UniProt: P35165

• KEGG entry: [3]

• E.C. number:

Additional information

• Expression of the SigX regulon in increased in ugtP mutants PubMed

Expression and regulation

• Operon: sigX-rsiX PubMed

• Expression browser: sigX PubMed

• Sigma factor: SigA PubMed, SigX PubMed

• Regulation:

• Regulatory mechanism: activated by YvrHb PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 156 PubMed

Biological materials

• Mutant:
  • 1A901 ( sigX::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

The SigX regulon

Thorsten Mascher, Anna-Barbara Hachmann, John D Helmann
Regulatory overlap and functional redundancy among Bacillus subtilis extracytoplasmic function sigma factors.
J Bacteriol: 2007, 189(19);6919-27
[PubMed:17675383] [WorldCat.org] [DOI] (P p)

M S Turner, J D Helmann
Mutations in multidrug efflux homologs, sugar isomerases, and antimicrobial biosynthesis genes differentially elevate activity of the sigma(X) and sigma(W) factors in Bacillus subtilis.
J Bacteriol: 2000, 182(18);5202-10
[PubMed:10960106] [WorldCat.org] [DOI] (P p)

X Huang, K L Fredrick, J D Helmann
Promoter recognition by Bacillus subtilis sigmaW: autoregulation and partial overlap with the sigmaX regulon.
J Bacteriol: 1998, 180(15);3765-70
[PubMed:9683469] [WorldCat.org] [DOI] (P p)