Difference between revisions of "SigF"

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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:'''
+
* '''Locus tag:''' BSU23450
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P07860 P07860]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P07860 P07860]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23450 BSU23450]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23450]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 10:03, 3 June 2009

  • Description: RNA polymerase forespore-specific (early) sigma factor SigF

Gene name sigF
Synonyms spoIIAC
Essential no
Product RNA polymerase forespore-specific
(early) sigma factor SigF
Function transcription of sporulation genes (late mother cell)
MW, pI 29 kDa, 5.078
Gene length, protein length 765 bp, 255 aa
Immediate neighbours spoVAA, spoIIAB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SigF context.gif
This image was kindly provided by SubtiList




The gene

Basic information

  • Locus tag: BSU23450

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sigma-70 factor family (according to Swiss-Prot)
  • Paralogous protein(s):

Genes controlled by SigF

dacF-spoIIAA-spoIIAB-sigF, sigG, pbpF, yphA-seaA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure: 1L0O (complex with SpoIIAB, Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

spoIIAA-spoIIAB-sigF

spoIIAA: SigH

  • Regulation: repressed by casamino acids PubMed , dacF: expressed during sporulation, spoIIAA: expressed early during sporulation
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed