sigF

168

[wiki|RNA polymerase] forespore-specific (early) [wiki|sigma factor] SigF

locus: BSU_23450

Molecular weight: 29.22 kDa

pI: 5.08

Protein length: 255 aa

Gene length: 768 bp

function: transcription of [wiki|sporulation] genes (early forespore)

product: [wiki|RNA polymerase] forespore-specific (early) [wiki|sigma factor] SigF

essential: no

synonyms: sigF, spoIIAC

List of homologs in different organisms, belongs to COG1191 (Galperin et al., 2021)

Gene

Coordinates: 2,443,429 → 2,444,196

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The protein

Protein family

• [wiki|Sigma-70 factor family] (according to UniProt)

Structure

• [PDB|1L0O] (complex with [protein|C8B867758330355F44C63CC284DC7AA3061FB9F1|spoIIAB], Geobacillus stearothermophilus) [pubmed|11955433]
• [AF|P07860]

Paralogous protein(s)

• [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]

Biological materials

Mutant

• BKE23450 (Δ[gene|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTCCACATCCATAACAAATC,  downstream forward: _UP4_TAGTCTGCAGTGCAGGCTAG
• BKK23450 (Δ[gene|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CTCCACATCCATAACAAATC,  downstream forward: _UP4_TAGTCTGCAGTGCAGGCTAG

labs

• [wiki|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

References

Reviews

• 20833318,31350897,35638784

Original Publications

• 10503549,8358793,7570023,9555886,3007937,8460142,9077448,10323866,10869437,7768847,14744853,8759874,10864495,1744043,16824103,11701124,1902463,8171000,1904527,15351644,15882622,8764398,8764397,9826498,9826499,2492512,1629150,12676949,7592342,11684022,8846916,1948031,8126438,3114419,12081956,9150232,15205443,9004507,8168129,2123551,8824593,19390092,17498739,8021191,1556084,15687200,12107147,18208527,10049355,23169620,21037003,20817675,21935351,25101664,11955433,28838935,29702640,38502707

The [wiki|SigF regulon]

• 16497325,26506528