SigD

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Gene name sigD
Synonyms flaB
Essential no
Product RNA polymerase sigma factor SigD
Function regulation of flagella, motility, chemotaxis and autolysis
Gene expression levels in SubtiExpress: sigD
Interactions involving this protein in SubtInteract: SigD
MW, pI 29 kDa, 5.167
Gene length, protein length 762 bp, 254 aa
Immediate neighbours cheD, swrB
Sequences Protein DNA DNA_with_flanks
Genetic context
SigD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SigD expression.png















Categories containing this gene/protein

transcription, sigma factors and their control, motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The SigD regulon

The gene

Basic information

  • Locus tag: BSU16470

Phenotypes of a mutant

  • Inactivation of alsR, sigD and sigW increases competitive fitness of Bacillus subtilis under non-sporulating conditions PubMed
  • mucoid phenotype due to the overproduction of poly-gamma-glutamate (because of the loss of motA-motB expression) PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sigma-70 factor family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: interaction with FlgM inhibits SigD activity PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • flgB promoter: repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • FLAG-tag construct: GP948 (ermC, based on pGP1087), available in the Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Sampriti Mukherjee, Daniel B Kearns
The structure and regulation of flagella in Bacillus subtilis.
Annu Rev Genet: 2014, 48;319-40
[PubMed:25251856] [WorldCat.org] [DOI] (I p)

Jan-Willem Veening, Oscar P Kuipers
Gene position within a long transcript as a determinant for stochastic switching in bacteria.
Mol Microbiol: 2010, 76(2);269-72
[PubMed:20233302] [WorldCat.org] [DOI] (I p)


The SigD regulon

Masakuni Serizawa, Hiroki Yamamoto, Hirotake Yamaguchi, Yasutaro Fujita, Kazuo Kobayashi, Naotake Ogasawara, Junichi Sekiguchi
Systematic analysis of SigD-regulated genes in Bacillus subtilis by DNA microarray and Northern blotting analyses.
Gene: 2004, 329;125-36
[PubMed:15033535] [WorldCat.org] [DOI] (P p)

L M Márquez, J D Helmann, E Ferrari, H M Parker, G W Ordal, M J Chamberlin
Studies of sigma D-dependent functions in Bacillus subtilis.
J Bacteriol: 1990, 172(6);3435-43
[PubMed:2111808] [WorldCat.org] [DOI] (P p)


Original publications