sigD

168

[wiki|RNA polymerase] [wiki|sigma factor] SigD

locus: BSU_16470

Molecular weight: 29.32 kDa

pI: 5.17

Protein length: 254 aa

Gene length: 765 bp

function: regulation of flagella, motility, chemotaxis and autolysis

product: [wiki|RNA polymerase] [wiki|sigma factor] SigD

essential: no

synonyms: sigD, flaB

List of homologs in different organisms, belongs to COG1191 (Galperin et al., 2021)

Gene

Coordinates: 1,716,493  1,717,257

Phenotypes of a mutant

• Inactivation of ''[gene|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]'', ''[gene|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]'' and ''[gene|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]'' increases competitive fitness of ''Bacillus subtilis'' under non-sporulating conditions [Pubmed|22344650]
• mucoid phenotype due to the overproduction of poly-gamma-glutamate (because of the loss of ''[gene|86C729C7D5ABEE519ADC4A893940600BBB655EF1|motA]-[gene|FE56753D061344B0F10A6523F49C5C7356AA40B6|motB]'' expression) [Pubmed|24296669]
• not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]
• defective in [category|SW.4.1.4|Swarming] motility [pubmed|35638827]

additional information

• the [gene|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD] gene is often inactivated during adaptation to plants [pubmed|37466402]

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The protein

Protein family

• [wiki|Sigma-70 factor family] (according to UniProt)

Structure

• [PDB|1RP3] ([protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]-[protein|F03144BF8A187C8931938A21433431B8961E8EE7|flgM] complex from ''Aqufex aeolicus'', 34% identity) [Pubmed|15068809]
• [AF|P10726]

Effectors of protein activity

• interaction with [protein|F03144BF8A187C8931938A21433431B8961E8EE7|flgM] inhibits SigD activity [Pubmed|10207036]

Biological materials

Mutant

• 1A716 ( ''sigD''::''cat''), [Pubmed|2832368], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A716&Search=1A716 BGSC]
• DS6420 (marker-less  in NCIB3610) [Pubmed|22329926]
• BKE16470 ([gene|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTTATCCCCCTAATAC,  downstream forward: _UP4_TAATGAATTTCATGGTTAGC
• BKK16470 ([gene|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16470 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTTATCCCCCTAATAC,  downstream forward: _UP4_TAATGAATTTCATGGTTAGC

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab

FLAG-tag construct

• GP948 (ermC, based on [wiki|pGP1087]), available in the [wiki|Jörg Stülke]'s lab

References

Reviews

• 20233302,25251856

Original Publications

• 36815589,26122431,22344650,22329926,21097624,22956758,21856853,8955328,7708689,9648743,8169223,9096206,15175317,2498284,16357223,3127378,26170408,9144176,18820022,7602586,2832368,8195064,11987133,14651647,9335309,9657996,8157612,10809682,8932324,20233303,17850253,10207036,16428420,24296669,25099370,24386445,26244495,15068809,29454936,35638827,37466402

The [wiki|SigD regulon]

• 15033535,2111808