Sfp/2

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  • Description: 4'-phosphopantetheinyl transferase (surfactin synthetase-activating enzyme), inactive pseudogene in strain 168

Gene name sfp/2
Synonyms sfp
Essential no
Product 4'-phosphopantetheinyl transferase
Function phosphopantetheinylates a serine residue in each
of the seven peptidyl carrier protein domains of the
first three subunits of surfactin synthetase (SrfAA-SrfAB-SrfAC) and AcpK
MW, pI - , -
Gene length, protein length 495 bp, -
Immediate neighbours sfp/1, yczE
Gene sequence (+200bp) Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context
Sfp context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
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Categories containing this gene/protein

miscellaneous metabolic pathways, biofilm formation, biosynthesis of antibacterial compounds, pseudogenes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU03570

Phenotypes of a mutant

No swarming motility on B medium. PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

  • This gene is a pseudogene in B. subtilis 168 due to a mutation that resulted in an internal frameshift. The second part of the pseudogene is sfp/2.
  • Correction of sfp, epsC, swrAA, and degQ as well as introduction of rapP from a plasmid present in NCIB3610 results in biofilm formation in B. subtilis 168 PubMed


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: CoA + apo-[peptidyl-carrier protein] = adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein] (according to Swiss-Prot)
  • Protein family: Gsp/sfp/hetI/acpT family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1QR0 (complex with CoA)
  • Swiss prot entry:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)

Original publications