secDF

168

preprotein translocase subunit (ATPase)

locus: BSU_27650

Molecular weight: 81.47 kDa

pI: 9.52

Protein length: 737 aa

Gene length: 2214 bp

function: protein secretion

product: preprotein translocase subunit (ATPase)

essential: no

synonyms: secDF, secF, secD

List of homologs in different organisms, belongs to COG0342 (Galperin et al., 2021)

Gene

Coordinates: 2,826,900 → 2,829,113

Phenotypes of a mutant

• reduced [wiki|protein secretion] [pubmed|32111210]
• defective in [category|SW.4.1.4|Swarming] motility (prolonged period of immotility prior to [category|SW.4.1.4|Swarming]) [pubmed|35638827]

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The protein

Protein family

• N-terminal part: SecD/SecF family (single member, according to UniProt)
• C-terminal part: SecD/SecF family (single member, according to UniProt)

Structure

• [PDB|5XAN] (from Deinococcus radiodurans, 32% identity) [pubmed|28467902]
• [AF|O32047]

[wiki|Localization]

• membrane [Pubmed|18763711]

Biological materials

Mutant

• MGNA-B519 (yrvB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1518 NBRP B. subtilis, Japan]
• SM-GN3 (''secDF-spc''), available in [wiki|Anne Galinier]'s and [wiki|Boris Görke]'s labs
• BKE27650 (Δ[gene|F0E7B6E000A5B06C9023C76F21551E754C8C45BA|secDF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATGTATATCCTCCCTT,  downstream forward: _UP4_TAAAAAATATCAGGCTGTCC
• BKK27650 (Δ[gene|F0E7B6E000A5B06C9023C76F21551E754C8C45BA|secDF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27650 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATGTATATCCTCCCTT,  downstream forward: _UP4_TAAAAAATATCAGGCTGTCC

References

Reviews

• 25212246

Original Publications

• 18763711,15995216,18179421,28467902,32111210,35638827,21562494,38474341